Gene description for Gnai3
Gene name guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3
Gene symbol Gnai3
Other names/aliases GIP3A
Species Rattus norvegicus
 Database cross references - Gnai3
ExoCarta ExoCarta_25643
Entrez Gene 25643
UniProt P08753  
 Gnai3 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Gnai3
Molecular Function
    GTPase activity GO:0003924 IBA
    signal transducer activity GO:0004871 IBA
    protein domain specific binding GO:0019904 IPI
    GTP binding GO:0005525 IEA
    protein binding GO:0005515 IPI
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    metal ion binding GO:0046872 IEA
    G-protein coupled serotonin receptor binding GO:0031821 IPI
    GTPase activating protein binding GO:0032794 IPI
Biological Process
    adenylate cyclase-modulating G-protein coupled receptor signaling pathway GO:0007188 IBA
    metabolic process GO:0008152 IEA
    vesicle fusion GO:0006906 IDA
    negative regulation of secretion GO:0051048 TAS
    regulation of heart contraction GO:0008016 ISO
    cell division GO:0051301 ISS
Subcellular Localization
    lysosomal membrane GO:0005765 ISO
    Golgi membrane GO:0000139 TAS
    membrane GO:0016020 ISO
    heterotrimeric G-protein complex GO:0005834 IBA
    zymogen granule GO:0042588 IDA
    cytoplasm GO:0005737 ISS
    membrane raft GO:0045121 IDA
    centrosome GO:0005813 ISS
    midbody GO:0030496 ISS
    plasma membrane GO:0005886 TAS
    extracellular exosome GO:0070062 ISO
    Golgi apparatus GO:0005794 ISO
 Experiment description of studies that identified Gnai3 in exosomes
1
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Gnai3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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