Gene description for Gphn
Gene name gephyrin
Gene symbol Gphn
Other names/aliases 5730552E08Rik
AI662856
BC027112
C230040D23
GPH
GPHRYN
geph
Species Mus musculus
 Database cross references - Gphn
ExoCarta ExoCarta_268566
Vesiclepedia VP_268566
Entrez Gene 268566
UniProt Q8BUV3  
 Gphn identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gphn
Molecular Function
    molybdopterin molybdotransferase activity GO:0061599 ISO
    protein complex scaffold GO:0032947 ISO
    receptor binding GO:0005102 ISO
    cytoskeletal protein binding GO:0008092 TAS
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
    molybdopterin adenylyltransferase activity GO:0061598 ISO
    transferase activity GO:0016740 IEA
    catalytic activity GO:0003824 IEA
    protein homodimerization activity GO:0042803 ISO
    ATP binding GO:0005524 IEA
    nucleotide binding GO:0000166 IEA
Biological Process
    protein homooligomerization GO:0051260 ISO
    establishment of protein localization GO:0045184 ISO
    molybdenum incorporation into molybdenum-molybdopterin complex GO:0018315 IBA
    Mo-molybdopterin cofactor biosynthetic process GO:0006777 ISO
    metabolic process GO:0008152 IEA
    establishment of synaptic specificity at neuromuscular junction GO:0007529 IMP
    molybdopterin cofactor biosynthetic process GO:0032324 IEA
    glycine receptor clustering GO:0072579 IMP
Subcellular Localization
    extrinsic component of plasma membrane GO:0019897 TAS
    intracellular GO:0005622 IDA
    plasma membrane GO:0005886 IEA
    inhibitory synapse GO:0060077 ISO
    cytoplasm GO:0005737 ISO
    cytoskeleton GO:0005856 IEA
    cell junction GO:0030054 IEA
    membrane GO:0016020 IEA
    postsynaptic membrane GO:0045211 IEA
    synapse GO:0045202 ISO
 Experiment description of studies that identified Gphn in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gphn
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Glrb  
Affinity Capture-Western Rattus norvegicus
View the network image/svg+xml
 Pathways in which Gphn is involved
PathwayEvidenceSource
Molybdenum cofactor biosynthesis IEA Reactome





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