Gene description for Adss
Gene name adenylosuccinate synthase
Gene symbol Adss
Other names/aliases Adss2
Species Rattus norvegicus
 Database cross references - Adss
ExoCarta ExoCarta_289276
Entrez Gene 289276
 Adss identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Adss
Molecular Function
    adenylosuccinate synthase activity GO:0004019 ISO
    magnesium ion binding GO:0000287 IEA
    GTP binding GO:0005525 IEA
Biological Process
    aspartate metabolic process GO:0006531 IDA
    IMP metabolic process GO:0046040 IDA
    'de novo' AMP biosynthetic process GO:0044208 IEA
    response to purine-containing compound GO:0014074 IEP
    AMP biosynthetic process GO:0006167 ISO
    cellular response to electrical stimulus GO:0071257 IEP
    purine nucleotide biosynthetic process GO:0006164 ISO
    response to ammonium ion GO:0060359 IEP
Subcellular Localization
    cytoplasm GO:0005737 ISO
    extracellular exosome GO:0070062 ISO
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Adss in exosomes
1
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Adss
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Adss is involved
No pathways found





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