Gene description for Clic4
Gene name chloride intracellular channel 4 (mitochondrial)
Gene symbol Clic4
Other names/aliases D0Jmb3
TU-74
mc3s5
mtCLIC
Species Mus musculus
 Database cross references - Clic4
ExoCarta ExoCarta_29876
Entrez Gene 29876
UniProt Q9QYB1  
 Clic4 identified in exosomes derived from the following tissue/cell type
Microglia 16081791    
 Gene ontology annotations for Clic4
Molecular Function
    chloride channel activity GO:0005254 ISO
    voltage-gated ion channel activity GO:0005244 IEA
    protein complex binding GO:0032403 ISO
    protein binding GO:0005515 IPI
Biological Process
    vacuolar acidification GO:0007035 IMP
    ion transport GO:0006811 IEA
    chloride transport GO:0006821 ISO
    keratinocyte differentiation GO:0030216 ISO
    cellular response to calcium ion GO:0071277 ISO
    negative regulation of cell migration GO:0030336 ISO
    transport GO:0006810 IEA
    fertilization GO:0009566 IMP
    angiogenesis GO:0001525 IMP
    branching morphogenesis of an epithelial tube GO:0048754 IMP
    regulation of ion transmembrane transport GO:0034765 IEA
    chloride transmembrane transport GO:1902476 ISO
    retina vasculature morphogenesis in camera-type eye GO:0061299 IMP
    endothelial cell morphogenesis GO:0001886 IMP
    apoptotic process GO:0006915 IEA
    multicellular organism growth GO:0035264 IMP
Subcellular Localization
    plasma membrane GO:0005886 ISO
    microvillus GO:0005902 ISO
    chloride channel complex GO:0034707 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    nucleus GO:0005634 IEA
    cell surface GO:0009986 ISO
    cell-cell junction GO:0005911 ISO
    apical part of cell GO:0045177 ISO
    cytosol GO:0005829 ISO
    microtubule cytoskeleton GO:0015630 ISO
    nuclear matrix GO:0016363 ISO
    cell GO:0005623 IMP
    intracellular GO:0005622 ISO
    centrosome GO:0005813 ISO
    midbody GO:0030496 ISO
    extracellular exosome GO:0070062 ISO
    membrane GO:0016020 IEA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 ISO
    vacuole GO:0005773 IMP
    integral component of membrane GO:0016021 ISS
    cytoplasmic vesicle GO:0031410 IEA
 Experiment description of studies that identified Clic4 in exosomes
1
Experiment ID 14
ISEV standards
✔
EM
EV Biophysical techniques
✔
RAB7|RAB11
EV Cytosolic markers
✔
CD9|CD63|LAMP1|LAMP2
EV Membrane markers
✔
DNM
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
 Protein-protein interactions for Clic4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Clic4 is involved
No pathways found





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