Gene description for Ube2m
Gene name ubiquitin-conjugating enzyme E2M
Gene symbol Ube2m
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Ube2m
ExoCarta ExoCarta_361509
Entrez Gene 361509
 Ube2m identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Ube2m
Molecular Function
    NEDD8 transferase activity GO:0019788 ISO
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin-protein transferase activity GO:0004842 ISO
    ribosomal S6-glutamic acid ligase activity GO:0018169 ISO
    molecular_function GO:0003674 ND
    ubiquitin protein ligase binding GO:0031625 IBA
Biological Process
    protein neddylation GO:0045116 ISO
    positive regulation of neuron apoptotic process GO:0043525 IMP
    cellular protein modification process GO:0006464 ISO
    protein ubiquitination GO:0016567 ISO
Subcellular Localization
    cellular_component GO:0005575 ND
    cytoplasm GO:0005737 IBA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Ube2m in exosomes
1
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ube2m
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ube2m is involved
No pathways found





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