Gene description for H2afz
Gene name H2A histone family, member Z
Gene symbol H2afz
Other names/aliases H2A.Z
H2a.z-1
Species Mus musculus
 Database cross references - H2afz
ExoCarta ExoCarta_51788
Entrez Gene 51788
UniProt P0C0S6  
 H2afz identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
Neural stem cells 25242146    
Oligodendrocytes 21136642    
 Gene ontology annotations for H2afz
Molecular Function
    protein heterodimerization activity GO:0046982 IEA
    RNA polymerase II core promoter sequence-specific DNA binding GO:0000979 ISO
    RNA polymerase II distal enhancer sequence-specific DNA binding GO:0000980 ISO
    nucleosomal DNA binding GO:0031492 ISO
    DNA binding GO:0003677 IEA
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 ISO
    chromatin DNA binding GO:0031490 ISO
Biological Process
    multicellular organismal development GO:0007275 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    cellular response to estradiol stimulus GO:0071392 ISO
    cellular response to insulin stimulus GO:0032869 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    nuclear euchromatin GO:0005719 ISO
    Barr body GO:0001740 ISA
    nuclear heterochromatin GO:0005720 ISO
    nucleosome GO:0000786 IEA
    nucleus GO:0005634 ISO
    chromosome GO:0005694 IEA
 Experiment description of studies that identified H2afz in exosomes
1
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 264
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 33
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for H2afz
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 h4-A  
Co-crystal Structure 8355
2 h2B  
Co-crystal Structure 8355
3 H2afz 51788
Co-crystal Structure Mus musculus
Co-crystal Structure Mus musculus
View the network image/svg+xml



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