Gene description for Nampt
Gene name nicotinamide phosphoribosyltransferase
Gene symbol Nampt
Other names/aliases 1110035O14Rik
AI314458
AI480535
NAmPRTase
Pbef
Pbef1
Visfatin
Species Mus musculus
 Database cross references - Nampt
ExoCarta ExoCarta_59027
Vesiclepedia VP_59027
Entrez Gene 59027
UniProt Q99KQ4  
 Nampt identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nampt
Molecular Function
    nicotinamide phosphoribosyltransferase activity GO:0047280 ISO
    drug binding GO:0008144 ISO
    nicotinate-nucleotide diphosphorylase (carboxylating) activity GO:0004514 IEA
    transferase activity GO:0016740 IEA
    cytokine activity GO:0005125 ISO
    transferase activity, transferring glycosyl groups GO:0016757 IEA
    protein homodimerization activity GO:0042803 ISO
Biological Process
    positive regulation of nitric-oxide synthase biosynthetic process GO:0051770 ISO
    circadian regulation of gene expression GO:0032922 IMP
    positive regulation of smooth muscle cell proliferation GO:0048661 ISO
    rhythmic process GO:0048511 IEA
    NAD biosynthetic process GO:0009435 IDA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    pyridine nucleotide biosynthetic process GO:0019363 IEA
Subcellular Localization
    extracellular region GO:0005576 IEA
    extracellular space GO:0005615 IEA
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 ISO
    nucleus GO:0005634 ISO
 Experiment description of studies that identified Nampt in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nampt
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Nampt is involved
PathwayEvidenceSource
Bmal1:Clock,Npas2 activates circadian gene expression TAS Reactome
Nicotinamide salvaging IEA Reactome





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