Gene description for Ago2
Gene name argonaute RISC catalytic component 2
Gene symbol Ago2
Other names/aliases Eif2c2
Species Rattus norvegicus
 Database cross references - Ago2
ExoCarta ExoCarta_59117
Entrez Gene 59117
UniProt Q9QZ81  
 Ago2 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Ago2
Molecular Function
    mRNA binding GO:0003729 ISO
    poly(A) RNA binding GO:0044822 ISO
    molecular_function GO:0003674 ND
    endoribonuclease activity GO:0004521 ISO
    endoribonuclease activity, cleaving siRNA-paired mRNA GO:0070551 ISS
    endoribonuclease activity, cleaving miRNA-paired mRNA GO:0090624 ISO
    core promoter binding GO:0001047 ISO
    RNA binding GO:0003723 ISO
    RNA polymerase II core binding GO:0000993 ISO
    metal ion binding GO:0046872 IEA
    siRNA binding GO:0035197 ISS
    miRNA binding GO:0035198 ISO
    pre-miRNA binding GO:0070883 ISO
    RNA 7-methylguanosine cap binding GO:0000340 ISS
    single-stranded RNA binding GO:0003727 ISO
    double-stranded RNA binding GO:0003725 ISO
Biological Process
    RNA secondary structure unwinding GO:0010501 ISO
    cell differentiation GO:0030154 NAS
    positive regulation of nuclear-transcribed mRNA poly(A) tail shortening GO:0060213 ISS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    negative regulation of translational initiation GO:0045947 ISS
    regulation of transcription, DNA-templated GO:0006355 IEA
    positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:1900153 ISS
    biological_process GO:0008150 ND
    post-embryonic development GO:0009791 ISO
    positive regulation of gene expression GO:0010628 ISO
    RNA phosphodiester bond hydrolysis, endonucleolytic GO:0090502 ISS
    production of miRNAs involved in gene silencing by miRNA GO:0035196 ISO
    siRNA loading onto RISC involved in RNA interference GO:0035087 ISO
    mRNA cleavage GO:0006379 ISO
    transcription, DNA-templated GO:0006351 IEA
    mRNA cleavage involved in gene silencing by miRNA GO:0035279 ISS
    negative regulation of translation involved in gene silencing by miRNA GO:0035278 ISS
    miRNA loading onto RISC involved in gene silencing by miRNA GO:0035280 ISO
    pre-miRNA processing GO:0031054 ISS
    mRNA cleavage involved in gene silencing by siRNA GO:0090625 ISO
    miRNA metabolic process GO:0010586 ISO
    gene silencing by RNA GO:0031047 ISS
Subcellular Localization
    membrane GO:0016020 ISO
    dendrite GO:0030425 ISO
    cytoplasm GO:0005737 ISO
    RISC-loading complex GO:0070578 ISS
    polysome GO:0005844 ISS
    nucleus GO:0005634 ISO
    ribonucleoprotein complex GO:0030529 ISS
    cellular_component GO:0005575 ND
    micro-ribonucleoprotein complex GO:0035068 ISS
    cytoplasmic mRNA processing body GO:0000932 ISO
    cytosol GO:0005829 ISO
    mRNA cap binding complex GO:0005845 ISS
    RISC complex GO:0016442 ISO
 Experiment description of studies that identified Ago2 in exosomes
1
Experiment ID 97
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ago2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ago2 is involved
No pathways found





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