Gene description for Pygl
Gene name phosphorylase, glycogen, liver
Gene symbol Pygl
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Pygl
ExoCarta ExoCarta_64035
Entrez Gene 64035
UniProt P09811  
 Pygl identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Pygl
Molecular Function
    drug binding GO:0008144 ISO
    ATP binding GO:0005524 ISO
    purine nucleobase binding GO:0002060 ISO
    protein homodimerization activity GO:0042803 IDA
    carbohydrate binding GO:0030246 IDA
    AMP binding GO:0016208 ISO
    pyridoxal phosphate binding GO:0030170 IBA
    glycogen phosphorylase activity GO:0008184 ISO
    vitamin binding GO:0019842 ISO
    bile acid binding GO:0032052 ISO
Biological Process
    necroptotic process GO:0070266 ISO
    glycogen metabolic process GO:0005977 ISO
    5-phosphoribose 1-diphosphate biosynthetic process GO:0006015 IMP
    glycogen catabolic process GO:0005980 IDA
    glucose homeostasis GO:0042593 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    plasma membrane GO:0005886 ISO
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Pygl in exosomes
1
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pygl
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pygl is involved
PathwayEvidenceSource
Glycogen breakdown (glycogenolysis) IEA Reactome





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