Gene description for Cul5
Gene name cullin 5
Gene symbol Cul5
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Cul5
ExoCarta ExoCarta_64624
Entrez Gene 64624
UniProt Q9JJ31  
 Cul5 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Cul5
Molecular Function
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin-protein transferase activity GO:0004842 TAS
    ubiquitin protein ligase binding GO:0031625 ISO
    vasopressin receptor activity GO:0005000 TAS
    protein heterodimerization activity GO:0046982 ISO
Biological Process
    protein polyubiquitination GO:0000209 TAS
    response to osmotic stress GO:0006970 IEP
    cerebral cortex radially oriented cell migration GO:0021799 ISO
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IBA
    cytosolic calcium ion homeostasis GO:0051480 IMP
    G-protein coupled receptor signaling pathway GO:0007186 TAS
    protein ubiquitination GO:0016567 ISO
    radial glia guided migration of Purkinje cell GO:0021942 ISO
Subcellular Localization
    nucleus GO:0005634 IDA
    plasma membrane GO:0005886 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IMP
    Cul5-RING ubiquitin ligase complex GO:0031466 TAS
 Experiment description of studies that identified Cul5 in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Cul5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Cul5 is involved
PathwayEvidenceSource
Antigen processing: Ubiquitination & Proteasome degradation IEA Reactome
Signaling by ERBB2 IEA Reactome





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