Experiment description of studies that identified Gm20899 in sEVs

1 Experiment ID 73 MISEV standards ✔ EM Biophysical techniques ✔ HSP70|HSP90 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 18494037     Organism Mus musculus Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes. Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ" Journal name ELEC Publication year 2008 Sample Embryonic fibroblasts Sample name NIH3T3 Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LCQ DECA] Western blotting

2 Experiment ID 210 MISEV standards ✔ EM Biophysical techniques ✔ CD81|FLOT1 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23260141     Organism Mus musculus Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration. Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL." Journal name Cell Publication year 2012 Sample Fibroblasts Sample name Normal-Fibroblasts (L cells) Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 214 MISEV standards ✔ EM Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23658846     Organism Mus musculus Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. Authors "Hassani K, Olivier M." Journal name PLoS Negl Trop Dis Publication year 2013 Sample Macrophages Sample name Leishmania-infected-Macrophage (J774A.1) Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 215 MISEV standards ✔ EM Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23658846     Organism Mus musculus Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. Authors "Hassani K, Olivier M." Journal name PLoS Negl Trop Dis Publication year 2013 Sample Macrophages Sample name LPS-treated-Macrophage (J774A.1) Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 216 MISEV standards ✔ EM Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23658846     Organism Mus musculus Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. Authors "Hassani K, Olivier M." Journal name PLoS Negl Trop Dis Publication year 2013 Sample Macrophages Sample name Normal-Macrophage (J774A.1) Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 264 MISEV standards ✘ Biophysical techniques ✔ CD63|CD9 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25242146     Organism Mus musculus Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino" Journal name Molecular Cell Publication year 2014 Sample Neural stem cells Sample name NPCs - Th2 treated Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.20 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 33 MISEV standards ✔ EM|IEM Biophysical techniques ✔ Alix|TSG101 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21136642     Organism Mus musculus Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons? Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J" Journal name PROTEOMICS_CL Publication year 2007 Sample Oligodendrocytes Sample name Oligodendrocytes Oli-neu Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.10-1.14 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [QTOF] Western blotting Immunoelectron Microscopy

8 Experiment ID 188 MISEV standards ✔ EM Biophysical techniques ✔ GAPDH|UCHL1|HSP90 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19351151     Organism Mus musculus Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells. Authors "Lee HS, Jeong J, Lee KJ." Journal name J Proteome Res Publication year 2009 Sample Pancreatic cells Sample name Pancreatic beta cell (NIT-1) Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for Gm20899

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Ywhae 22627 Affinity Capture-MS Mus musculus View the network

Pathways in which Gm20899 is involved

No pathways found