Gene description for GPC6
Gene name glypican 6
Gene symbol GPC6
Other names/aliases OMIMD1
Species Homo sapiens
 Database cross references - GPC6
ExoCarta ExoCarta_10082
Vesiclepedia VP_10082
Entrez Gene 10082
HGNC 4454
MIM 604404
UniProt Q9Y625  
 GPC6 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for GPC6
Molecular Function
    protein binding GO:0005515 IPI
    coreceptor activity GO:0015026 NAS
Biological Process
    regulation of signal transduction GO:0009966 IEA
    cell migration GO:0016477 IBA
    cell migration GO:0016477 IDA
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 IBA
Subcellular Localization
    extracellular region GO:0005576 IEA
    nucleus GO:0005634 HDA
    Golgi lumen GO:0005796 TAS
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IBA
    lysosomal lumen GO:0043202 TAS
    synapse GO:0045202 IBA
    collagen-containing extracellular matrix GO:0062023 IEA
    side of membrane GO:0098552 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified GPC6 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
9
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GPC6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CAMKV 79012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 MRPS7 51081
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 LRRIQ1  
Affinity Capture-MS Homo sapiens
4 MECP2 4204
Affinity Capture-MS Homo sapiens
5 GPC4 2239
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 TMEM30A 55754
Affinity Capture-MS Homo sapiens
7 HADHA 3030
Affinity Capture-MS Homo sapiens
8 HPN  
Affinity Capture-MS Homo sapiens
9 CHST8 64377
Affinity Capture-MS Homo sapiens
10 SRP9 6726
Affinity Capture-MS Homo sapiens
11 DLX4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 CLEC12B  
Affinity Capture-MS Homo sapiens
13 OGFOD3 79701
Affinity Capture-MS Homo sapiens
14 PLTP 5360
Affinity Capture-MS Homo sapiens
15 GPR183  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 SCARA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 MLX  
Affinity Capture-MS Homo sapiens
18 ST14 6768
Affinity Capture-MS Homo sapiens
19 PTPRS 5802
Reconstituted Complex Homo sapiens
20 FGF1  
Affinity Capture-MS Homo sapiens
21 LRRTM3  
Affinity Capture-MS Homo sapiens
22 TMPRSS13  
Affinity Capture-MS Homo sapiens
23 TULP3 7289
Affinity Capture-MS Homo sapiens
24 ZACN  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GPC6 is involved
PathwayEvidenceSource
A tetrasaccharide linker sequence is required for GAG synthesis TAS Reactome
Attachment and Entry TAS Reactome
Attachment and Entry IEA Reactome
Chondroitin sulfate/dermatan sulfate metabolism TAS Reactome
Defective B3GALT6 causes EDSP2 and SEMDJL1 TAS Reactome
Defective B3GAT3 causes JDSSDHD TAS Reactome
Defective B4GALT7 causes EDS, progeroid type TAS Reactome
Defective EXT1 causes exostoses 1, TRPS2 and CHDS TAS Reactome
Defective EXT2 causes exostoses 2 TAS Reactome
Disease TAS Reactome
Disease IEA Reactome
Diseases associated with glycosaminoglycan metabolism TAS Reactome
Diseases of glycosylation TAS Reactome
Diseases of metabolism TAS Reactome
Early SARS-CoV-2 Infection Events TAS Reactome
Early SARS-CoV-2 Infection Events IEA Reactome
Glycosaminoglycan metabolism TAS Reactome
Glycosaminoglycan metabolism IEA Reactome
Heparan sulfate/heparin (HS-GAG) metabolism TAS Reactome
Heparan sulfate/heparin (HS-GAG) metabolism IEA Reactome
HS-GAG biosynthesis TAS Reactome
HS-GAG biosynthesis IEA Reactome
HS-GAG degradation TAS Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Metabolism TAS Reactome
Metabolism IEA Reactome
Metabolism of carbohydrates TAS Reactome
Metabolism of carbohydrates IEA Reactome
Metabolism of fat-soluble vitamins TAS Reactome
Metabolism of vitamins and cofactors TAS Reactome
Respiratory syncytial virus (RSV) attachment and entry TAS Reactome
Respiratory Syncytial Virus Infection Pathway TAS Reactome
Retinoid metabolism and transport TAS Reactome
RSV-host interactions TAS Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV Infections IEA Reactome
SARS-CoV-2 Infection TAS Reactome
SARS-CoV-2 Infection IEA Reactome
Sensory Perception TAS Reactome
Viral Infection Pathways TAS Reactome
Viral Infection Pathways IEA Reactome
Visual phototransduction TAS Reactome





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