Gene description for MSLN
Gene name mesothelin
Gene symbol MSLN
Other names/aliases MPF
SMRP
Species Homo sapiens
 Database cross references - MSLN
ExoCarta ExoCarta_10232
Vesiclepedia VP_10232
Entrez Gene 10232
HGNC 7371
MIM 601051
UniProt Q13421  
 MSLN identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for MSLN
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    cell adhesion GO:0007155 NAS
    cell-matrix adhesion GO:0007160 IBA
Subcellular Localization
    extracellular region GO:0005576 TAS
    endoplasmic reticulum lumen GO:0005788 TAS
    Golgi apparatus GO:0005794 IEA
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IBA
    membrane GO:0016020 NAS
    side of membrane GO:0098552 IEA
 Experiment description of studies that identified MSLN in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MSLN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 ANLN 54443
Affinity Capture-MS Homo sapiens
3 RING1 6015
Affinity Capture-MS Homo sapiens
4 RSG1  
Two-hybrid Homo sapiens
5 PRG2  
Affinity Capture-MS Homo sapiens
6 LYPD3 27076
Affinity Capture-MS Homo sapiens
7 HLA-G 3135
Affinity Capture-MS Homo sapiens
8 GOLGA8A 23015
Affinity Capture-MS Homo sapiens
9 BTF3 689
Affinity Capture-MS Homo sapiens
10 GGH 8836
Affinity Capture-MS Homo sapiens
11 DDX31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 LARP1B 55132
Affinity Capture-MS Homo sapiens
13 MPV17 4358
Co-fractionation Homo sapiens
14 NDUFB5 4711
Co-fractionation Homo sapiens
15 BSPRY 54836
Affinity Capture-MS Homo sapiens
16 ECEL1  
Affinity Capture-MS Homo sapiens
17 CBLN4  
Affinity Capture-MS Homo sapiens
18 ZDHHC23  
Affinity Capture-MS Homo sapiens
19 NCR3  
Affinity Capture-MS Homo sapiens
20 IL5RA  
Affinity Capture-MS Homo sapiens
21 RALA 5898
Co-fractionation Homo sapiens
22 FKBP14  
Affinity Capture-MS Homo sapiens
23 SFTPC  
Affinity Capture-MS Homo sapiens
24 APEX1 328
Affinity Capture-RNA Homo sapiens
25 MRPL1  
Co-fractionation Homo sapiens
26 NDUFB9 4715
Co-fractionation Homo sapiens
27 RNMTL1  
Co-fractionation Homo sapiens
28 SDF2L1 23753
Affinity Capture-MS Homo sapiens
29 PPY  
Affinity Capture-MS Homo sapiens
30 ATP6V1A 523
Co-fractionation Homo sapiens
31 PCGF2 7703
Affinity Capture-MS Homo sapiens
32 GXYLT1 283464
Affinity Capture-MS Homo sapiens
33 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
34 MRPL28 10573
Co-fractionation Homo sapiens
35 LYPD4  
Affinity Capture-MS Homo sapiens
36 LAMTOR3 8649
Co-fractionation Homo sapiens
37 ZPBP2  
Affinity Capture-MS Homo sapiens
38 ZBTB43  
Co-fractionation Homo sapiens
39 MRPL16  
Co-fractionation Homo sapiens
View the network image/svg+xml



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