Gene description for SMYD5
Gene name SMYD family member 5
Gene symbol SMYD5
Other names/aliases NN8-4AG
RAI15
RRG1
ZMYND23
Species Homo sapiens
 Database cross references - SMYD5
ExoCarta ExoCarta_10322
Vesiclepedia VP_10322
Entrez Gene 10322
HGNC 16258
UniProt Q6GMV2  
 SMYD5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
 Gene ontology annotations for SMYD5
Molecular Function
    protein binding GO:0005515 IPI
    protein-lysine N-methyltransferase activity GO:0016279 IDA
    histone H4K20 methyltransferase activity GO:0042799 IBA
    histone H4K20 methyltransferase activity GO:0042799 ISS
    metal ion binding GO:0046872 IEA
    histone H4K20 trimethyltransferase activity GO:0140943 IEA
    histone H3K36 trimethyltransferase activity GO:0140955 ISS
Biological Process
    methylation GO:0032259 IEA
    negative regulation of gene expression, epigenetic GO:0045814 IBA
    negative regulation of gene expression, epigenetic GO:0045814 ISS
    transposable element silencing by heterochromatin formation GO:0141005 IMP
    positive regulation of cytoplasmic translational elongation GO:1900249 IDA
    regulation of stem cell division GO:2000035 ISS
    regulation of stem cell differentiation GO:2000736 IMP
    regulation of stem cell differentiation GO:2000736 ISS
Subcellular Localization
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified SMYD5 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for SMYD5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HIST1H3A 8350
Biochemical Activity Homo sapiens
2 ZNF430  
Affinity Capture-MS Homo sapiens
3 WWOX 51741
Affinity Capture-MS Homo sapiens
4 UBA52 7311
Affinity Capture-MS Homo sapiens
5 HIST1H4A 8359
Biochemical Activity Homo sapiens
6 HIST1H2BB 3018
Biochemical Activity Homo sapiens
7 USP11 8237
Affinity Capture-Western Homo sapiens
8 TUB  
Affinity Capture-MS Homo sapiens
9 POLR2A 5430
Co-localization Homo sapiens
10 MYO1C 4641
Co-fractionation Homo sapiens
11 NXF1 10482
Affinity Capture-RNA Homo sapiens
12 DCD 117159
Affinity Capture-MS Homo sapiens
13 ZNF446  
Affinity Capture-MS Homo sapiens
14 MOV10 4343
Affinity Capture-RNA Homo sapiens
15 TMEM132A 54972
Affinity Capture-MS Homo sapiens
16 RPA3 6119
Proximity Label-MS Homo sapiens
17 CDK9 1025
Co-localization Homo sapiens
18 KIF5B 3799
Co-fractionation Homo sapiens
19 HIST1H1A 3024
Biochemical Activity Homo sapiens
20 ZNF587  
Two-hybrid Homo sapiens
21 TKFC 26007
Affinity Capture-MS Homo sapiens
22 LMNA 4000
Proximity Label-MS Homo sapiens
23 ZNF417  
Two-hybrid Homo sapiens
24 XRCC5 7520
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SMYD5 is involved
No pathways found





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