Gene description for KLF2
Gene name Kruppel-like factor 2
Gene symbol KLF2
Other names/aliases LKLF
Species Homo sapiens
 Database cross references - KLF2
ExoCarta ExoCarta_10365
Vesiclepedia VP_10365
Entrez Gene 10365
HGNC 6347
MIM 602016
UniProt Q9Y5W3  
 KLF2 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for KLF2
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IMP
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA binding GO:0003677 IMP
    DNA-binding transcription factor activity GO:0003700 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    cell morphogenesis GO:0000902 IEA
    in utero embryonic development GO:0001701 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    negative regulation of interleukin-6 production GO:0032715 IEA
    multicellular organism growth GO:0035264 IEA
    epigenetic regulation of gene expression GO:0040029 IEA
    vasodilation GO:0042311 IMP
    erythrocyte maturation GO:0043249 IEA
    positive regulation of nitric oxide biosynthetic process GO:0045429 IDA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    positive regulation of retinoic acid receptor signaling pathway GO:0048386 IDA
    positive regulation of protein metabolic process GO:0051247 IGI
    type I pneumocyte differentiation GO:0060509 IEA
    cellular response to hydrogen peroxide GO:0070301 IEA
    cellular response to interleukin-1 GO:0071347 IEA
    cellular response to tumor necrosis factor GO:0071356 IEA
    cellular response to cycloheximide GO:0071409 IEA
    cellular response to fluid shear stress GO:0071498 IMP
    cellular response to laminar fluid shear stress GO:0071499 IMP
    cellular stress response to acid chemical GO:0097533 IMP
    cellular response to peptide GO:1901653 IEA
    negative regulation of sprouting angiogenesis GO:1903671 IMP
Subcellular Localization
    chromatin GO:0000785 IMP
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IEA
 Experiment description of studies that identified KLF2 in sEVs
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for KLF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GATA6  
Two-hybrid Homo sapiens
2 KAT2B  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 SMURF1 57154
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 GATA5  
Two-hybrid Homo sapiens
5 MYC  
Affinity Capture-Western Homo sapiens
6 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
7 CREBBP  
Co-localization Homo sapiens
8 WWP1 11059
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 EP300 2033
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 PSME3 10197
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which KLF2 is involved
No pathways found





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