Gene description for Acly
Gene name ATP citrate lyase
Gene symbol Acly
Other names/aliases A730098H14Rik
AW538652
Species Mus musculus
 Database cross references - Acly
ExoCarta ExoCarta_104112
Vesiclepedia VP_104112
Entrez Gene 104112
UniProt Q91V92  
 Acly identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Acly
Molecular Function
    ATP citrate synthase activity GO:0003878 IBA
    ATP citrate synthase activity GO:0003878 IDA
    ATP citrate synthase activity GO:0003878 ISO
    ATP citrate synthase activity GO:0003878 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    acetyl-CoA metabolic process GO:0006084 ISO
    acetyl-CoA biosynthetic process GO:0006085 IBA
    acetyl-CoA biosynthetic process GO:0006085 ISO
    acetyl-CoA biosynthetic process GO:0006085 ISS
    citrate metabolic process GO:0006101 ISO
    citrate metabolic process GO:0006101 ISS
    oxaloacetate metabolic process GO:0006107 ISO
    oxaloacetate metabolic process GO:0006107 ISS
    fatty acid biosynthetic process GO:0006633 IBA
    fatty acid biosynthetic process GO:0006633 ISO
    lipid biosynthetic process GO:0008610 ISO
    lipid biosynthetic process GO:0008610 ISS
Subcellular Localization
    nucleoplasm GO:0005654 ISO
    mitochondrion GO:0005739 HDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
 Experiment description of studies that identified Acly in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Acly
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kpna2 16647
Co-fractionation Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Eed  
Affinity Capture-MS Mus musculus
5 Traf3  
Proximity Label-MS Mus musculus
6 Tfe3  
Affinity Capture-MS Mus musculus
7 Srrt 83701
Co-fractionation Mus musculus
8 Fancd2  
Affinity Capture-MS Mus musculus
9 Ywhae 22627
Affinity Capture-MS Mus musculus
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