Gene description for SPON1
Gene name spondin 1, extracellular matrix protein
Gene symbol SPON1
Other names/aliases VSGP/F-spondin
f-spondin
Species Homo sapiens
 Database cross references - SPON1
ExoCarta ExoCarta_10418
Vesiclepedia VP_10418
Entrez Gene 10418
HGNC 11252
MIM 604989
UniProt Q9HCB6  
 SPON1 identified in sEVs derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SPON1
Molecular Function
    extracellular matrix structural constituent GO:0005201 RCA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    LBD domain binding GO:0050693 IEA
Biological Process
    cell adhesion GO:0007155 IBA
    positive regulation of protein processing GO:0010954 IEA
    protein processing GO:0016485 IEA
    negative regulation of amyloid-beta formation GO:1902430 IEA
    positive regulation of amyloid precursor protein catabolic process GO:1902993 IEA
Subcellular Localization
    extracellular space GO:0005615 HDA
    endoplasmic reticulum lumen GO:0005788 TAS
    extracellular matrix GO:0031012 IBA
    collagen-containing extracellular matrix GO:0062023 HDA
 Experiment description of studies that identified SPON1 in sEVs
1
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SPON1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZMYND19  
Affinity Capture-MS Homo sapiens
2 CUL3 8452
Affinity Capture-MS Homo sapiens
3 AHCYL1 10768
Affinity Capture-MS Homo sapiens
4 DPY19L3  
Affinity Capture-MS Homo sapiens
5 RMND5B  
Affinity Capture-MS Homo sapiens
6 MDFI  
Two-hybrid Homo sapiens
7 GID4  
Affinity Capture-MS Homo sapiens
8 SLURP1  
Affinity Capture-MS Homo sapiens
9 WDR26 80232
Affinity Capture-MS Homo sapiens
10 ARMC8 25852
Affinity Capture-MS Homo sapiens
11 RANBP9 10048
Affinity Capture-MS Homo sapiens
12 GID8 54994
Affinity Capture-MS Homo sapiens
13 SULF2 55959
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 CYSRT1 375791
Two-hybrid Homo sapiens
15 RANBP10 57610
Affinity Capture-MS Homo sapiens
16 MEOX2  
Two-hybrid Homo sapiens
17 RMND5A 64795
Affinity Capture-MS Homo sapiens
18 ST14 6768
Affinity Capture-MS Homo sapiens
19 NEDD8-MDP1 100528064
Affinity Capture-MS Homo sapiens
20 MKLN1  
Affinity Capture-MS Homo sapiens
21 C5orf22  
Affinity Capture-MS Homo sapiens
22 TMPRSS13  
Affinity Capture-MS Homo sapiens
23 MAEA 10296
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here