Gene description for Scrib
Gene name scribbled homolog (Drosophila)
Gene symbol Scrib
Other names/aliases AI118201
CRIB
Crc
SCRIB1
Scrb1
mKIAA0147
vartul
Species Mus musculus
 Database cross references - Scrib
ExoCarta ExoCarta_105782
Vesiclepedia VP_105782
Entrez Gene 105782
UniProt Q80U72  
 Scrib identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Scrib
Molecular Function
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IBA
    signaling adaptor activity GO:0035591 IEA
    signaling adaptor activity GO:0035591 ISO
Biological Process
    establishment of T cell polarity GO:0001768 IDA
    neural tube closure GO:0001843 IGI
    neural tube closure GO:0001843 IMP
    neural tube closure GO:0001843 ISO
    positive regulation of receptor recycling GO:0001921 IEA
    positive regulation of receptor recycling GO:0001921 ISO
    auditory receptor cell morphogenesis GO:0002093 IGI
    epithelial cell morphogenesis GO:0003382 TAS
    protein localization GO:0008104 IMP
    cell population proliferation GO:0008283 ISO
    cell population proliferation GO:0008283 ISS
    positive regulation of epithelial cell migration GO:0010634 ISS
    epithelial structure maintenance GO:0010669 ISS
    synaptic vesicle targeting GO:0016080 IMP
    morphogenesis of embryonic epithelium GO:0016331 IMP
    cell migration GO:0016477 IBA
    cell migration GO:0016477 IMP
    cell migration GO:0016477 ISO
    cochlear nucleus development GO:0021747 IGI
    polarized epithelial cell differentiation GO:0030859 IEA
    polarized epithelial cell differentiation GO:0030859 ISO
    positive regulation of type II interferon production GO:0032729 IMP
    establishment of apical/basal cell polarity GO:0035089 ISO
    establishment of apical/basal cell polarity GO:0035089 ISS
    post-anal tail morphogenesis GO:0036342 IGI
    post-anal tail morphogenesis GO:0036342 IMP
    wound healing GO:0042060 IGI
    positive regulation of apoptotic process GO:0043065 ISO
    positive regulation of apoptotic process GO:0043065 ISS
    receptor clustering GO:0043113 IBA
    astrocyte cell migration GO:0043615 IEA
    astrocyte cell migration GO:0043615 ISO
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 IBA
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 TAS
    negative regulation of mitotic cell cycle GO:0045930 ISO
    negative regulation of mitotic cell cycle GO:0045930 ISS
    negative regulation of translational initiation GO:0045947 ISO
    negative regulation of activated T cell proliferation GO:0046007 IMP
    synaptic vesicle endocytosis GO:0048488 IDA
    synaptic vesicle endocytosis GO:0048488 IMP
    camera-type eye morphogenesis GO:0048593 TAS
    positive chemotaxis GO:0050918 IMP
    positive chemotaxis GO:0050918 ISO
    auditory receptor cell stereocilium organization GO:0060088 IGI
    inner ear receptor cell stereocilium organization GO:0060122 IMP
    apoptotic process involved in morphogenesis GO:0060561 ISO
    apoptotic process involved in morphogenesis GO:0060561 ISS
    mammary gland duct morphogenesis GO:0060603 IMP
    protein localization to adherens junction GO:0071896 IEA
    protein localization to adherens junction GO:0071896 ISO
    activation of GTPase activity GO:0090630 ISO
    activation of GTPase activity GO:0090630 ISS
    cell-cell adhesion GO:0098609 IBA
    cell-cell adhesion GO:0098609 ISO
    cell-cell adhesion GO:0098609 ISS
    neurotransmitter receptor transport, endosome to postsynaptic membrane GO:0098887 IBA
    neurotransmitter receptor transport, endosome to postsynaptic membrane GO:0098887 IEA
    neurotransmitter receptor transport, endosome to postsynaptic membrane GO:0098887 ISO
    neurotransmitter receptor transport postsynaptic membrane to endosome GO:0098968 IBA
    neurotransmitter receptor transport postsynaptic membrane to endosome GO:0098968 ISO
    vesicle-mediated transport in synapse GO:0099003 ISO
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 IEA
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 ISO
Subcellular Localization
    immunological synapse GO:0001772 IDA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    cell-cell junction GO:0005911 ISO
    cell-cell junction GO:0005911 ISS
    adherens junction GO:0005912 IBA
    adherens junction GO:0005912 ISO
    adherens junction GO:0005912 ISS
    postsynaptic density GO:0014069 IBA
    postsynaptic density GO:0014069 ISO
    basolateral plasma membrane GO:0016323 IBA
    basolateral plasma membrane GO:0016323 IDA
    apical plasma membrane GO:0016324 TAS
    lamellipodium GO:0030027 IEA
    cell junction GO:0030054 ISO
    cell leading edge GO:0031252 ISO
    cell leading edge GO:0031252 ISS
    Scrib-APC-beta-catenin complex GO:0034750 IEA
    Scrib-APC-beta-catenin complex GO:0034750 ISO
    myelin sheath abaxonal region GO:0035748 IDA
    presynaptic membrane GO:0042734 ISO
    presynaptic membrane GO:0042734 ISS
    cell-cell contact zone GO:0044291 IDA
    postsynaptic membrane GO:0045211 IBA
    postsynaptic membrane GO:0045211 ISO
    postsynaptic membrane GO:0045211 ISS
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
    extrinsic component of postsynaptic density membrane GO:0099147 IEA
    extrinsic component of postsynaptic density membrane GO:0099147 ISO
 Experiment description of studies that identified Scrib in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Scrib
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fxr1 14359
Affinity Capture-MS Mus musculus
2 Tsc1  
Affinity Capture-MS Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Fmr1  
Affinity Capture-MS Mus musculus
5 Pde4dip  
Affinity Capture-MS Mus musculus
6 Kcna3  
Affinity Capture-MS Mus musculus
7 Kctd13  
Affinity Capture-MS Mus musculus
8 Mycbp2 105689
Affinity Capture-MS Mus musculus
9 Phlpp1  
Co-fractionation Mus musculus
10 Vangl2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
11 Cdh1 12550
Co-fractionation Mus musculus
12 Apc  
Affinity Capture-Western Mus musculus
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