Gene description for Unc5b
Gene name unc-5 homolog B (C. elegans)
Gene symbol Unc5b
Other names/aliases 6330415E02Rik
A630020F16
D10Bwg0792e
Unc5h2
Species Mus musculus
 Database cross references - Unc5b
ExoCarta ExoCarta_107449
Vesiclepedia VP_107449
Entrez Gene 107449
UniProt Q8K1S3  
 Unc5b identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Unc5b
Molecular Function
    netrin receptor activity GO:0005042 IBA
    netrin receptor activity GO:0005042 IEA
    netrin receptor activity GO:0005042 ISO
    protein binding GO:0005515 IPI
Biological Process
    angiogenesis GO:0001525 IEA
    apoptotic process GO:0006915 IEA
    axon guidance GO:0007411 ISO
    axon guidance GO:0007411 ISO
    anterior/posterior axon guidance GO:0033564 IBA
    anterior/posterior axon guidance GO:0033564 IMP
    netrin-activated signaling pathway GO:0038007 IEA
    negative regulation of neuron apoptotic process GO:0043524 IEA
    negative regulation of neuron apoptotic process GO:0043524 ISO
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 ISO
    negative regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001240 IEA
    negative regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001240 ISO
    positive regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001241 ISO
Subcellular Localization
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
    membrane raft GO:0045121 IEA
    membrane raft GO:0045121 ISO
 Experiment description of studies that identified Unc5b in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Unc5b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nphs2  
Affinity Capture-MS Mus musculus
2 Ppp2r1a 51792
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
3 Ppp2r1b 73699
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
4 Ppp2ca 19052
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Unc5b is involved
No pathways found





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