Gene description for BTN3A2
Gene name butyrophilin, subfamily 3, member A2
Gene symbol BTN3A2
Other names/aliases BT3.2
BTF4
BTN3.2
CD277
Species Homo sapiens
 Database cross references - BTN3A2
ExoCarta ExoCarta_11118
Vesiclepedia VP_11118
Entrez Gene 11118
HGNC 1139
MIM 613594
UniProt P78410  
 BTN3A2 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for BTN3A2
Molecular Function
    signaling receptor binding GO:0005102 IBA
    protein binding GO:0005515 IPI
Biological Process
    regulation of cytokine production GO:0001817 IBA
    T cell mediated immunity GO:0002456 IMP
    positive regulation of type II interferon production GO:0032729 IMP
    T cell receptor signaling pathway GO:0050852 IBA
Subcellular Localization
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IBA
    membrane GO:0016020 HDA
 Experiment description of studies that identified BTN3A2 in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for BTN3A2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UFSP2 55325
Affinity Capture-MS Homo sapiens
2 CLDN2 9075
Two-hybrid Homo sapiens
3 PEX13  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 NSF 4905
Affinity Capture-MS Homo sapiens
5 ATP5G1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 KRAS 3845
Proximity Label-MS Homo sapiens
7 TMEM245 23731
Affinity Capture-MS Homo sapiens
8 BTN3A1 11119
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 RAB35 11021
Affinity Capture-MS Homo sapiens
10 CCDC68  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 MCAM 4162
Proximity Label-MS Homo sapiens
12 C1orf27 54953
Affinity Capture-MS Homo sapiens
13 BTNL9  
Affinity Capture-MS Homo sapiens
14 BTN3A3 10384
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 DDX58 23586
Affinity Capture-RNA Homo sapiens
16 SLC39A11  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which BTN3A2 is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Butyrophilin (BTN) family interactions TAS Reactome
Immune System TAS Reactome





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