Gene description for Hadh
Gene name hydroxyacyl-CoA dehydrogenase
Gene symbol Hadh
Other names/aliases Hadhsc
Species Rattus norvegicus
 Database cross references - Hadh
ExoCarta ExoCarta_113965
Vesiclepedia VP_113965
Entrez Gene 113965
UniProt Q9WVK7  
 Hadh identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Hadh
Molecular Function
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 IBA
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 IMP
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 ISO
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 ISS
    identical protein binding GO:0042802 ISO
    identical protein binding GO:0042802 ISS
    NAD+ binding GO:0070403 ISO
    NAD+ binding GO:0070403 ISS
Biological Process
    fatty acid beta-oxidation GO:0006635 IBA
    fatty acid beta-oxidation GO:0006635 IEA
    fatty acid beta-oxidation GO:0006635 IMP
    fatty acid beta-oxidation GO:0006635 ISO
    fatty acid beta-oxidation GO:0006635 ISS
    spermatogenesis GO:0007283 IEA
    response to xenobiotic stimulus GO:0009410 IDA
    response to hormone GO:0009725 IEP
    response to activity GO:0014823 IDA
    cell differentiation GO:0030154 IEA
    response to insulin GO:0032868 IEP
    negative regulation of insulin secretion GO:0046676 IMP
    regulation of insulin secretion GO:0050796 ISO
    regulation of insulin secretion GO:0050796 ISS
    positive regulation of cold-induced thermogenesis GO:0120162 ISO
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    mitochondrial matrix GO:0005759 IEA
 Experiment description of studies that identified Hadh in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Hadh
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
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