Gene description for Supt16
Gene name suppressor of Ty 16
Gene symbol Supt16
Other names/aliases Cdc68
Fact140
Spt16
Supt16h
Species Mus musculus
 Database cross references - Supt16
ExoCarta ExoCarta_114741
Vesiclepedia VP_114741
Entrez Gene 114741
UniProt Q920B9  
 Supt16 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Supt16
Molecular Function
    nucleosome binding GO:0031491 IBA
Biological Process
    DNA replication GO:0006260 IEA
    DNA repair GO:0006281 IEA
    nucleosome assembly GO:0006334 NAS
    nucleosome disassembly GO:0006337 ISO
    transcription elongation by RNA polymerase II GO:0006368 IBA
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    FACT complex GO:0035101 IBA
    FACT complex GO:0035101 ISO
 Experiment description of studies that identified Supt16 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Supt16
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Paf1 54624
Co-fractionation Mus musculus
2 Rasd1  
Two-hybrid Mus musculus
3 Tnik  
Affinity Capture-MS Mus musculus
4 Pou5f1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
5 Rtf1  
Co-fractionation Mus musculus
6 Smarcc2  
Co-fractionation Mus musculus
7 Twist1  
Proximity Label-MS Mus musculus
8 Fmr1  
Affinity Capture-MS Mus musculus
9 H2afx 15270
Affinity Capture-MS Mus musculus
10 Smarca2  
Co-fractionation Mus musculus
11 Zbtb7b  
Affinity Capture-MS Mus musculus
12 Ctr9  
Co-fractionation Mus musculus
13 Atg5  
Affinity Capture-MS Mus musculus
14 Nanog  
Affinity Capture-MS Mus musculus
15 Rnf20  
Co-fractionation Mus musculus
16 Tfcp2l1  
Affinity Capture-MS Mus musculus
17 Qrich1  
Co-fractionation Mus musculus
18 Leo1  
Co-fractionation Mus musculus
19 Eed  
Affinity Capture-MS Mus musculus
20 Baz1b 22385
Co-fractionation Mus musculus
21 Atxn1  
Affinity Capture-MS Mus musculus
22 Atg16l1  
Affinity Capture-MS Mus musculus
23 Uhrf1  
Affinity Capture-MS Mus musculus
24 Fancd2  
Affinity Capture-MS Mus musculus
25 Nek9  
Co-fractionation Mus musculus
View the network image/svg+xml



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