Gene description for Csnk2a1
Gene name casein kinase 2, alpha 1 polypeptide
Gene symbol Csnk2a1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Csnk2a1
ExoCarta ExoCarta_116549
Vesiclepedia VP_116549
Entrez Gene 116549
UniProt P19139  
 Csnk2a1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Csnk2a1
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IEA
    protein serine/threonine kinase activity GO:0004674 ISO
    protein serine/threonine kinase activity GO:0004674 ISS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    ATP binding GO:0005524 IEA
    beta-catenin binding GO:0008013 ISO
    kinase activity GO:0016301 ISO
    protein phosphatase regulator activity GO:0019888 ISO
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    identical protein binding GO:0042802 ISO
    ribonucleoprotein complex binding GO:0043021 IDA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    double-strand break repair GO:0006302 IBA
    double-strand break repair GO:0006302 ISO
    double-strand break repair GO:0006302 ISS
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 ISO
    protein phosphorylation GO:0006468 ISO
    protein phosphorylation GO:0006468 ISS
    apoptotic process GO:0006915 IEA
    DNA damage response GO:0006974 ISO
    positive regulation of cell population proliferation GO:0008284 ISO
    positive regulation of cell population proliferation GO:0008284 ISS
    Wnt signaling pathway GO:0016055 IEA
    negative regulation of translation GO:0017148 ISO
    peptidyl-serine phosphorylation GO:0018105 ISO
    peptidyl-serine phosphorylation GO:0018105 ISS
    peptidyl-threonine phosphorylation GO:0018107 ISO
    cerebral cortex development GO:0021987 IEP
    positive regulation of Wnt signaling pathway GO:0030177 ISO
    positive regulation of Wnt signaling pathway GO:0030177 ISS
    positive regulation of cell growth GO:0030307 ISO
    positive regulation of cell growth GO:0030307 ISS
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 ISO
    response to testosterone GO:0033574 IEP
    positive regulation of protein catabolic process GO:0045732 ISO
    positive regulation of protein catabolic process GO:0045732 ISS
    protein autophosphorylation GO:0046777 ISO
    rhythmic process GO:0048511 IEA
    protein stabilization GO:0050821 ISO
    regulation of cell cycle GO:0051726 IBA
    symbiont-mediated disruption of host cell PML body GO:0075342 ISO
    liver regeneration GO:0097421 IEP
    regulation of protein localization to plasma membrane GO:1903076 IGI
    positive regulation of aggrephagy GO:1905337 ISO
    positive regulation of aggrephagy GO:1905337 ISS
    regulation of chromosome separation GO:1905818 ISO
    regulation of chromosome separation GO:1905818 ISS
    negative regulation of double-strand break repair via homologous recombination GO:2000042 ISO
    negative regulation of double-strand break repair via homologous recombination GO:2000042 ISS
    negative regulation of cysteine-type endopeptidase activity GO:2000117 ISO
    negative regulation of cysteine-type endopeptidase activity GO:2000117 ISS
Subcellular Localization
    chromatin GO:0000785 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    cytosol GO:0005829 IBA
    protein kinase CK2 complex GO:0005956 IBA
    protein kinase CK2 complex GO:0005956 ISO
    PML body GO:0016605 ISO
    PcG protein complex GO:0031519 ISO
    PcG protein complex GO:0031519 ISS
    Sin3-type complex GO:0070822 ISO
 Experiment description of studies that identified Csnk2a1 in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 101
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Csnk2a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mib1  
Affinity Capture-MS Rattus norvegicus
Affinity Capture-MS Rattus norvegicus
2 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
3 Grin2b  
Biochemical Activity Rattus norvegicus
View the network image/svg+xml
 Pathways in which Csnk2a1 is involved
PathwayEvidenceSource
Autophagy IEA Reactome
Axon guidance IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Chaperonin-mediated protein folding IEA Reactome
Condensation of Prometaphase Chromosomes IEA Reactome
Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding IEA Reactome
Developmental Biology IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
Glycerophospholipid biosynthesis IEA Reactome
Intracellular signaling by second messengers IEA Reactome
L1CAM interactions IEA Reactome
M Phase IEA Reactome
Macroautophagy IEA Reactome
Metabolism IEA Reactome
Metabolism of lipids IEA Reactome
Metabolism of proteins IEA Reactome
Mitophagy IEA Reactome
Mitotic Prometaphase IEA Reactome
Nervous system development IEA Reactome
Phospholipid metabolism IEA Reactome
PIP3 activates AKT signaling IEA Reactome
Protein folding IEA Reactome
PTEN Regulation IEA Reactome
Receptor Mediated Mitophagy IEA Reactome
Regulation of PTEN stability and activity IEA Reactome
Regulation of TP53 Activity IEA Reactome
Regulation of TP53 Activity through Phosphorylation IEA Reactome
RNA Polymerase II Transcription IEA Reactome
RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known IEA Reactome
Selective autophagy IEA Reactome
Signal Transduction IEA Reactome
Signal transduction by L1 IEA Reactome
Signaling by WNT IEA Reactome
Synthesis of PC IEA Reactome
TCF dependent signaling in response to WNT IEA Reactome
Transcriptional regulation by RUNX1 IEA Reactome
Transcriptional Regulation by TP53 IEA Reactome
WNT mediated activation of DVL IEA Reactome





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