Gene description for Hnrnpk
Gene name heterogeneous nuclear ribonucleoprotein K
Gene symbol Hnrnpk
Other names/aliases Csbp
Hnrpk
Species Rattus norvegicus
 Database cross references - Hnrnpk
ExoCarta ExoCarta_117282
Vesiclepedia VP_117282
Entrez Gene 117282
UniProt P61980  
 Hnrnpk identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Hnrnpk
Molecular Function
    cis-regulatory region sequence-specific DNA binding GO:0000987 IDA
    DNA binding GO:0003677 IEA
    double-stranded DNA binding GO:0003690 IDA
    single-stranded DNA binding GO:0003697 IDA
    RNA binding GO:0003723 IEA
    RNA binding GO:0003723 ISO
    mRNA binding GO:0003729 IBA
    mRNA 3'-UTR binding GO:0003730 IDA
    protein binding GO:0005515 IPI
    lamin binding GO:0005521 IPI
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 IPI
    protein domain specific binding GO:0019904 ISO
    pre-mRNA 3'-splice site binding GO:0030628 IDA
    heat shock protein binding GO:0031072 IPI
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    actinin binding GO:0042805 IPI
    ribonucleoprotein complex binding GO:0043021 IDA
    ribonucleoprotein complex binding GO:0043021 IPI
    ATPase binding GO:0051117 IPI
    mRNA CDS binding GO:1990715 IDA
    C-rich single-stranded DNA binding GO:1990829 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    ovarian follicle development GO:0001541 IMP
    kidney development GO:0001822 IEP
    liver development GO:0001889 IEP
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    regulation of transcription by RNA polymerase II GO:0006357 IMP
    mRNA processing GO:0006397 IEA
    acute-phase response GO:0006953 IEP
    peripheral nervous system development GO:0007422 IEP
    RNA splicing GO:0008380 IEA
    male gonad development GO:0008584 IEP
    negative regulation of gene expression GO:0010629 IMP
    positive regulation of neuron projection development GO:0010976 IMP
    regulation of low-density lipoprotein particle clearance GO:0010988 IEA
    regulation of low-density lipoprotein particle clearance GO:0010988 ISO
    response to activity GO:0014823 IEP
    cerebellum development GO:0021549 IEP
    hippocampus development GO:0021766 IEP
    cerebral cortex development GO:0021987 IDA
    cerebral cortex development GO:0021987 IEP
    lung development GO:0030324 IEP
    regulatory ncRNA-mediated heterochromatin formation GO:0031048 IEA
    regulatory ncRNA-mediated heterochromatin formation GO:0031048 ISO
    positive regulation of myelination GO:0031643 IMP
    cellular response to insulin stimulus GO:0032869 IDA
    cellular response to insulin stimulus GO:0032869 IEP
    positive regulation of RNA splicing GO:0033120 IMP
    camera-type eye development GO:0043010 IEP
    negative regulation of apoptotic process GO:0043066 IEA
    negative regulation of apoptotic process GO:0043066 IMP
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of DNA-templated transcription GO:0045892 IEA
    negative regulation of DNA-templated transcription GO:0045892 ISO
    negative regulation of DNA-templated transcription GO:0045892 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    regulation of mRNA splicing, via spliceosome GO:0048024 IBA
    negative regulation of mRNA splicing, via spliceosome GO:0048025 ISO
    positive regulation of receptor-mediated endocytosis GO:0048260 IEA
    positive regulation of receptor-mediated endocytosis GO:0048260 ISO
    thymus development GO:0048538 IEP
    modulation of chemical synaptic transmission GO:0050804 IDA
    modulation of chemical synaptic transmission GO:0050804 IMP
    positive regulation of synaptic transmission GO:0050806 IMP
    random inactivation of X chromosome GO:0060816 IEA
    random inactivation of X chromosome GO:0060816 ISO
    positive regulation of dendritic spine development GO:0060999 IMP
    cellular response to amino acid stimulus GO:0071230 IEP
    cellular response to lead ion GO:0071284 IEP
    cellular response to glucose stimulus GO:0071333 IEP
    cellular response to rapamycin GO:0072752 IEP
    positive regulation of synapse maturation GO:0090129 IMP
    regulation of postsynapse organization GO:0099175 IDA
    regulation of postsynapse organization GO:0099175 IMP
    positive regulation of long-term synaptic potentiation GO:1900273 IMP
    response to salt GO:1902074 IEP
    regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:1902165 ISO
    positive regulation of dendrite extension GO:1903861 IMP
    cellular response to forskolin GO:1904322 IDA
    positive regulation of low-density lipoprotein receptor activity GO:1905599 ISO
    positive regulation of protein localization to cell surface GO:2000010 IMP
    negative regulation of branching morphogenesis of a nerve GO:2000173 IMP
Subcellular Localization
    chromatin GO:0000785 IEA
    chromatin GO:0000785 ISO
    chromatin GO:0000785 ISS
    podosome GO:0002102 IEA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    spliceosomal complex GO:0005681 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cell cortex GO:0005938 IDA
    cytoplasmic stress granule GO:0010494 ISO
    protein-DNA complex GO:0032993 IDA
    cell projection GO:0042995 IEA
    dendritic spine GO:0043197 IDA
    axon terminus GO:0043679 IDA
    anchoring junction GO:0070161 IEA
    catalytic step 2 spliceosome GO:0071013 IEA
    catalytic step 2 spliceosome GO:0071013 ISO
    postsynapse GO:0098794 IDA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Hnrnpk in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hnrnpk
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
2 Park7 117287
Affinity Capture-MS Rattus norvegicus
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