Gene description for Cdh2
Gene name cadherin 2
Gene symbol Cdh2
Other names/aliases CDHN
N-cadherin
Ncad
Species Mus musculus
 Database cross references - Cdh2
ExoCarta ExoCarta_12558
Vesiclepedia VP_12558
Entrez Gene 12558
UniProt P15116  
 Cdh2 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cdh2
Molecular Function
    RNA binding GO:0003723 IDA
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    beta-catenin binding GO:0008013 IBA
    beta-catenin binding GO:0008013 IEA
    beta-catenin binding GO:0008013 ISO
    enzyme binding GO:0019899 IPI
    protein kinase binding GO:0019901 IPI
    protein kinase binding GO:0019901 ISO
    protein phosphatase binding GO:0019903 IPI
    identical protein binding GO:0042802 IPI
    protein-containing complex binding GO:0044877 ISO
    alpha-catenin binding GO:0045294 IEA
    alpha-catenin binding GO:0045294 ISO
    gamma-catenin binding GO:0045295 IEA
    gamma-catenin binding GO:0045295 ISO
    cadherin binding GO:0045296 IBA
    nitric-oxide synthase binding GO:0050998 ISO
    protein tyrosine kinase binding GO:1990782 ISO
Biological Process
    cell morphogenesis GO:0000902 IBA
    type B pancreatic cell development GO:0003323 IMP
    cell-cell junction assembly GO:0007043 IBA
    cell-cell junction assembly GO:0007043 IMP
    cell adhesion GO:0007155 IDA
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IDA
    heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules GO:0007157 IDA
    synapse assembly GO:0007416 IBA
    synapse assembly GO:0007416 ISO
    glial cell differentiation GO:0010001 IDA
    neural crest cell development GO:0014032 IMP
    calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules GO:0016339 IBA
    calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules GO:0016339 IDA
    calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules GO:0016339 ISO
    cell migration GO:0016477 IBA
    cell migration GO:0016477 IDA
    telencephalon development GO:0021537 IGI
    cerebral cortex development GO:0021987 IGI
    regulation of myelination GO:0031641 ISO
    regulation of protein localization GO:0032880 ISO
    adherens junction organization GO:0034332 IBA
    regulation of Rho protein signal transduction GO:0035023 ISO
    positive regulation of MAPK cascade GO:0043410 IGI
    cell-cell adhesion mediated by cadherin GO:0044331 IBA
    cell-cell adhesion mediated by cadherin GO:0044331 IDA
    blood vessel morphogenesis GO:0048514 IMP
    brain morphogenesis GO:0048854 IGI
    homeostasis of number of cells GO:0048872 IGI
    regulation of axonogenesis GO:0050770 ISO
    modulation of chemical synaptic transmission GO:0050804 ISO
    striated muscle cell differentiation GO:0051146 IGI
    regulation of synaptic transmission, glutamatergic GO:0051966 ISO
    radial glial cell differentiation GO:0060019 IGI
    neuroepithelial cell differentiation GO:0060563 IGI
    regulation of oligodendrocyte progenitor proliferation GO:0070445 IGI
    protein localization to plasma membrane GO:0072659 IDA
    negative regulation of canonical Wnt signaling pathway GO:0090090 IGI
    mesenchymal cell migration GO:0090497 IMP
    synaptic vesicle clustering GO:0097091 IMP
    neuroligin clustering involved in postsynaptic membrane assembly GO:0097118 IMP
    neuronal stem cell population maintenance GO:0097150 IDA
    cell-cell adhesion GO:0098609 IDA
    cell-cell adhesion GO:0098609 ISO
    regulation of postsynaptic density protein 95 clustering GO:1902897 IMP
    positive regulation of synaptic vesicle clustering GO:2000809 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    cell-cell junction GO:0005911 IDA
    cell-cell junction GO:0005911 ISO
    adherens junction GO:0005912 IBA
    adherens junction GO:0005912 IDA
    adherens junction GO:0005912 ISO
    fascia adherens GO:0005916 IDA
    fascia adherens GO:0005916 ISO
    cell surface GO:0009986 IDA
    postsynaptic density GO:0014069 IBA
    intercalated disc GO:0014704 IBA
    intercalated disc GO:0014704 IDA
    intercalated disc GO:0014704 ISO
    membrane GO:0016020 ISO
    basolateral plasma membrane GO:0016323 IDA
    apical plasma membrane GO:0016324 IDA
    apicolateral plasma membrane GO:0016327 IDA
    catenin complex GO:0016342 IBA
    catenin complex GO:0016342 IEA
    catenin complex GO:0016342 ISO
    lamellipodium GO:0030027 IBA
    lamellipodium GO:0030027 IDA
    lamellipodium GO:0030027 ISO
    cell junction GO:0030054 ISO
    desmosome GO:0030057 IDA
    desmosome GO:0030057 ISO
    T-tubule GO:0030315 ISO
    cortical actin cytoskeleton GO:0030864 IEA
    cortical actin cytoskeleton GO:0030864 ISO
    protein-containing complex GO:0032991 ISO
    sarcolemma GO:0042383 IDA
    neuron projection GO:0043005 IBA
    plasma membrane raft GO:0044853 IDA
    apical part of cell GO:0045177 IBA
    synapse GO:0045202 IDA
    synapse GO:0045202 ISO
    presynaptic active zone membrane GO:0048787 IBA
    presynaptic active zone membrane GO:0048787 ISO
    presynapse GO:0098793 IEA
    glutamatergic synapse GO:0098978 ISO
    postsynaptic specialization membrane GO:0099634 IBA
    postsynaptic specialization membrane GO:0099634 ISO
 Experiment description of studies that identified Cdh2 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1101
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 1102
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
4
Experiment ID 1103
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cdh2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fxr1 14359
Affinity Capture-MS Mus musculus
2 Tnik  
Affinity Capture-MS Mus musculus
3 Cyfip2 76884
Affinity Capture-MS Mus musculus
4 Pde4dip  
Affinity Capture-MS Mus musculus
5 Gja1 14609
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
6 Ptprk 19272
Co-fractionation Mus musculus
7 Ctnnb1 12387
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
8 Axin1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
9 Ctnnd1 12388
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
10 Psen1  
Affinity Capture-Western Mus musculus
11 Kctd13  
Affinity Capture-MS Mus musculus
12 Pex5l  
Affinity Capture-MS Mus musculus
13 Csk 12988
Affinity Capture-Western Mus musculus
14 Nphp4  
Affinity Capture-MS Mus musculus
15 Grin2b  
Affinity Capture-Western Mus musculus
16 Ptprq  
Affinity Capture-Western Mus musculus
17 Arhgap32  
Affinity Capture-Western Mus musculus
18 Kif5c  
Affinity Capture-Western Mus musculus
19 Lrp6  
Affinity Capture-Western Mus musculus
Phenotypic Suppression Mus musculus
20 Grin1  
Affinity Capture-Western Mus musculus
21 Wnt3a  
Affinity Capture-Western Mus musculus
22 Kifap3  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
23 Lrp5  
Affinity Capture-Western Mus musculus
Phenotypic Suppression Mus musculus
Affinity Capture-Western Mus musculus
24 Ndn  
Two-hybrid Mus musculus
25 Pkp4  
Co-fractionation Mus musculus
26 Jup 16480
Affinity Capture-Western Mus musculus
27 Cdh11  
Co-fractionation Mus musculus
28 Kif3b  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
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