Experiment description of studies that identified PODN in sEVs

1 Experiment ID 494 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Osteoarthritic cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 496 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Healthy cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 126 MISEV standards ✘ Biophysical techniques ✔ GAPDH Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim" Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

Protein-protein interactions for PODN

Protein Interactor ExoCarta ID Identification method PubMed Species 1 FRAS1 80144 Affinity Capture-MS Homo sapiens 2 CNPY4 245812 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 3 SPSB3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 4 DCAF8 50717 Affinity Capture-MS Homo sapiens 5 IL17RA 23765 Affinity Capture-MS Homo sapiens 6 TUBB8 347688 Affinity Capture-MS Homo sapiens 7 HSPA5 3309 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 8 GALNS 2588 Affinity Capture-MS Homo sapiens 9 IDS   Affinity Capture-MS Homo sapiens 10 LRRC6   Two-hybrid Homo sapiens 11 CBWD1 55871 Affinity Capture-MS Homo sapiens 12 LRP6 4040 Affinity Capture-MS Homo sapiens 13 LRP5 4041 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 14 RCN2 5955 Affinity Capture-MS Homo sapiens 15 PASK   Affinity Capture-MS Homo sapiens 16 OS9 10956 Affinity Capture-MS Homo sapiens 17 RCN1 5954 Affinity Capture-MS Homo sapiens View the network

Pathways in which PODN is involved

No pathways found