Gene description for Ddx6
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
Gene symbol Ddx6
Other names/aliases 1110001P04Rik
E230023J21Rik
HLR2
mRCK/P54
p54
rck
Species Mus musculus
 Database cross references - Ddx6
ExoCarta ExoCarta_13209
Vesiclepedia VP_13209
Entrez Gene 13209
UniProt P54823  
 Ddx6 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ddx6
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    RNA binding GO:0003723 IEA
    RNA helicase activity GO:0003724 IEA
    mRNA binding GO:0003729 IBA
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    negative regulation of translation GO:0017148 IBA
    negative regulation of translation GO:0017148 ISO
    negative regulation of translation GO:0017148 ISS
    viral RNA genome packaging GO:0019074 IEA
    viral RNA genome packaging GO:0019074 ISO
    stem cell population maintenance GO:0019827 IMP
    neuron differentiation GO:0030182 IMP
    P-body assembly GO:0033962 IBA
    P-body assembly GO:0033962 ISO
    P-body assembly GO:0033962 ISO
    P-body assembly GO:0033962 ISS
    stress granule assembly GO:0034063 IBA
    miRNA-mediated gene silencing by inhibition of translation GO:0035278 ISO
    miRNA-mediated gene silencing by inhibition of translation GO:0035278 ISS
    negative regulation of neuron differentiation GO:0045665 IMP
    chromatin looping GO:0140588 IEA
Subcellular Localization
    heterochromatin GO:0000792 ISO
    P-body GO:0000932 IBA
    P-body GO:0000932 IDA
    P-body GO:0000932 ISO
    outer dense fiber GO:0001520 ISO
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    adherens junction GO:0005912 ISO
    cytoplasmic stress granule GO:0010494 IBA
    cytoplasmic stress granule GO:0010494 ISO
    cytoplasmic stress granule GO:0010494 ISS
    RISC complex GO:0016442 ISO
    chromatoid body GO:0033391 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 IDA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    concave side of sperm head GO:0061830 ISO
    sperm annulus GO:0097227 ISO
 Experiment description of studies that identified Ddx6 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ddx6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rpl28 19943
Co-fractionation Mus musculus
2 Psmc2 19181
Co-fractionation Mus musculus
3 Rplp0 11837
Co-fractionation Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
5 Ybx1 22608
Co-fractionation Mus musculus
6 Fmr1  
Co-fractionation Mus musculus
7 Lsm14a  
Co-fractionation Mus musculus
8 Eed  
Affinity Capture-MS Mus musculus
9 Poldip3  
Co-fractionation Mus musculus
10 Urm1  
Co-fractionation Mus musculus
11 Atg12  
Positive Genetic Mus musculus
12 Rptor  
Co-fractionation Mus musculus
13 Gigyf2  
Co-fractionation Mus musculus
14 Rps6 20104
Co-fractionation Mus musculus
15 Ncl 17975
Co-fractionation Mus musculus
16 Rps4x 20102
Co-fractionation Mus musculus
17 Fancd2  
Affinity Capture-MS Mus musculus
18 Ywhae 22627
Affinity Capture-MS Mus musculus
19 Kctd13  
Affinity Capture-MS Mus musculus
20 Rock2  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Ddx6 is involved
PathwayEvidenceSource
Deadenylation-dependent mRNA decay IEA Reactome
Metabolism of RNA IEA Reactome
mRNA decay by 5' to 3' exoribonuclease IEA Reactome





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