Gene description for Eif2s1
Gene name eukaryotic translation initiation factor 2, subunit 1 alpha
Gene symbol Eif2s1
Other names/aliases 0910001O23Rik
2410026C18Rik
35kDa
Eif2a
eIF2alpha
Species Mus musculus
 Database cross references - Eif2s1
ExoCarta ExoCarta_13665
Vesiclepedia VP_13665
Entrez Gene 13665
UniProt Q6ZWX6  
 Eif2s1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eif2s1
Molecular Function
    RNA binding GO:0003723 IEA
    translation initiation factor activity GO:0003743 IBA
    translation initiation factor activity GO:0003743 ISO
    translation initiation factor activity GO:0003743 ISS
    protein binding GO:0005515 IPI
    ribosome binding GO:0043022 IBA
    ribosome binding GO:0043022 ISO
    ribosome binding GO:0043022 ISS
Biological Process
    mitophagy GO:0000423 ISO
    mitophagy GO:0000423 ISS
    translation GO:0006412 IDA
    translational initiation GO:0006413 IBA
    translational initiation GO:0006413 IEA
    translational initiation GO:0006413 ISO
    negative regulation of translational initiation in response to stress GO:0032057 IDA
    stress granule assembly GO:0034063 IMP
    cellular response to amino acid starvation GO:0034198 IDA
    cellular response to oxidative stress GO:0034599 IMP
    cellular response to heat GO:0034605 IEA
    cellular response to heat GO:0034605 ISO
    cellular response to UV GO:0034644 IDA
    response to endoplasmic reticulum stress GO:0034976 IDA
    response to endoplasmic reticulum stress GO:0034976 ISO
    regulation of translation in response to endoplasmic reticulum stress GO:0036490 IEA
    regulation of translation in response to endoplasmic reticulum stress GO:0036490 ISO
    PERK-mediated unfolded protein response GO:0036499 ISO
    PERK-mediated unfolded protein response GO:0036499 ISS
    regulation of translational initiation in response to stress GO:0043558 IDA
    protein autophosphorylation GO:0046777 IDA
    HRI-mediated signaling GO:0140468 ISO
    HRI-mediated signaling GO:0140468 ISS
    response to kainic acid GO:1904373 IEA
    response to manganese-induced endoplasmic reticulum stress GO:1990737 IEA
    positive regulation of type B pancreatic cell apoptotic process GO:2000676 IEA
    positive regulation of type B pancreatic cell apoptotic process GO:2000676 ISO
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    eukaryotic translation initiation factor 2 complex GO:0005850 IBA
    eukaryotic translation initiation factor 2 complex GO:0005850 ISO
    eukaryotic translation initiation factor 2 complex GO:0005850 ISS
    cytoplasmic stress granule GO:0010494 IDA
    eukaryotic 48S preinitiation complex GO:0033290 IBA
    translation initiation ternary complex GO:0044207 IEA
    translation initiation ternary complex GO:0044207 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    glial limiting end-foot GO:0097451 IEA
    glial limiting end-foot GO:0097451 ISO
 Experiment description of studies that identified Eif2s1 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eif2s1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PCBP3 54039
Two-hybrid Homo sapiens
2 Cebpa  
Affinity Capture-MS Mus musculus
3 Sstr3  
Proximity Label-MS Mus musculus
4 EIF4ENIF1 56478
Two-hybrid Homo sapiens
5 Eed  
Affinity Capture-MS Mus musculus
6 Upf1 19704
Co-fractionation Mus musculus
7 Tfe3  
Affinity Capture-MS Mus musculus
8 Srsf6 67996
Two-hybrid Mus musculus
9 Set 56086
Affinity Capture-MS Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
10 Cirbp  
Co-fractionation Mus musculus
11 Fancd2  
Affinity Capture-MS Mus musculus
12 Kctd13  
Affinity Capture-MS Mus musculus
13 Eif2a  
Co-fractionation Mus musculus
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