Gene description for CSF3
Gene name colony stimulating factor 3 (granulocyte)
Gene symbol CSF3
Other names/aliases C17orf33
CSF3OS
GCSF
Species Homo sapiens
 Database cross references - CSF3
ExoCarta ExoCarta_1440
Vesiclepedia VP_1440
Entrez Gene 1440
HGNC 2438
MIM 138970
UniProt P09919  
 CSF3 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for CSF3
Molecular Function
    cytokine activity GO:0005125 IBA
    cytokine activity GO:0005125 IDA
    cytokine activity GO:0005125 NAS
    granulocyte colony-stimulating factor receptor binding GO:0005130 IBA
    growth factor activity GO:0008083 IBA
    enzyme binding GO:0019899 IPI
Biological Process
    immune response GO:0006955 IEA
    positive regulation of cell population proliferation GO:0008284 IBA
    cytokine-mediated signaling pathway GO:0019221 NAS
    positive regulation of actin filament polymerization GO:0030838 IDA
    granulocyte differentiation GO:0030851 NAS
    granulocyte colony-stimulating factor signaling pathway GO:0038158 IDA
    response to ethanol GO:0045471 IEA
    positive regulation of myeloid cell differentiation GO:0045639 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IDA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IDA
    cellular response to lipopolysaccharide GO:0071222 IEA
    cellular response to cytokine stimulus GO:0071345 IDA
    regulation of actin filament organization GO:0110053 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    lysosomal lumen GO:0043202 TAS
    endocytic vesicle lumen GO:0071682 TAS
 Experiment description of studies that identified CSF3 in sEVs
1
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Antibody array
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for CSF3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COL14A1 7373
Affinity Capture-MS Homo sapiens
2 TBX3 6926
Two-hybrid Homo sapiens
3 PTPRJ 5795
Affinity Capture-MS Homo sapiens
4 PTPRG 5793
Affinity Capture-MS Homo sapiens
5 AHNAK2 113146
Affinity Capture-MS Homo sapiens
6 MYO18A 399687
Affinity Capture-MS Homo sapiens
7 SCARB1 949
Affinity Capture-MS Homo sapiens
8 PARVG 64098
Two-hybrid Homo sapiens
9 NRN1  
Affinity Capture-MS Homo sapiens
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