Gene description for Gdi1
Gene name guanosine diphosphate (GDP) dissociation inhibitor 1
Gene symbol Gdi1
Other names/aliases GDIA
GDIalpha
Species Mus musculus
 Database cross references - Gdi1
ExoCarta ExoCarta_14567
Vesiclepedia VP_14567
Entrez Gene 14567
UniProt P50396  
 Gdi1 identified in sEVs derived from the following tissue/cell type
Oligodendrocytes 21136642    
 Gene ontology annotations for Gdi1
Molecular Function
    GDP-dissociation inhibitor activity GO:0005092 ISO
    Rab GDP-dissociation inhibitor activity GO:0005093 IBA
    Rab GDP-dissociation inhibitor activity GO:0005093 ISO
    Rab GDP-dissociation inhibitor activity GO:0005093 ISS
    GTPase activator activity GO:0005096 IEA
    small GTPase binding GO:0031267 IEA
    small GTPase binding GO:0031267 ISO
Biological Process
    protein transport GO:0015031 IEA
    vesicle-mediated transport GO:0016192 IBA
    Rab protein signal transduction GO:0032482 ISS
    positive regulation of toll-like receptor signaling pathway GO:0034123 IEA
    positive regulation of toll-like receptor signaling pathway GO:0034123 ISO
    positive regulation of axon extension GO:0045773 IEA
    positive regulation of axon extension GO:0045773 ISO
    negative regulation of axonogenesis GO:0050771 ISO
    negative regulation of axonogenesis GO:0050771 ISS
    response to calcium ion GO:0051592 IEA
    response to calcium ion GO:0051592 ISO
    negative regulation of protein targeting to membrane GO:0090315 ISO
    negative regulation of protein targeting to membrane GO:0090315 ISS
Subcellular Localization
    cytoplasm GO:0005737 ISO
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    Golgi apparatus GO:0005794 IEA
    cytosol GO:0005829 IBA
    axon GO:0030424 IEA
    axon GO:0030424 ISO
    midbody GO:0030496 IEA
    midbody GO:0030496 ISO
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
    myelin sheath GO:0043209 HDA
    myelin sheath GO:0043209 ISO
    presynaptic cytosol GO:0099523 IEA
    presynaptic cytosol GO:0099523 ISO
 Experiment description of studies that identified Gdi1 in sEVs
1
Experiment ID 33
MISEV standards
EM|IEM
Biophysical techniques
Alix|TSG101
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Gdi1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rab1 19324
Affinity Capture-Western Mus musculus
2 Gnai2 14678
Co-fractionation Mus musculus
3 Kcna3  
Affinity Capture-MS Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Krt27  
Co-fractionation Mus musculus
6 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
7 Mapt  
Affinity Capture-MS Mus musculus
8 Sgta 52551
Co-fractionation Mus musculus
9 Unk  
Affinity Capture-RNA Mus musculus
10 Scrn1  
Co-fractionation Mus musculus
11 Kcnma1  
Affinity Capture-MS Mus musculus
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