Gene description for CCBE1
Gene name collagen and calcium binding EGF domains 1
Gene symbol CCBE1
Other names/aliases HKLLS1
Species Homo sapiens
 Database cross references - CCBE1
ExoCarta ExoCarta_147372
Vesiclepedia VP_147372
Entrez Gene 147372
HGNC 29426
MIM 612753
UniProt Q6UXH8  
 CCBE1 identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for CCBE1
Molecular Function
    protease binding GO:0002020 IPI
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    collagen binding GO:0005518 IDA
Biological Process
    lymphangiogenesis GO:0001946 IMP
    lymphangiogenesis GO:0001946 ISS
    sprouting angiogenesis GO:0002040 ISS
    respiratory system process GO:0003016 IEA
    positive regulation of vascular endothelial growth factor production GO:0010575 IDA
    positive regulation of endothelial cell migration GO:0010595 IEA
    positive regulation of protein processing GO:0010954 IDA
    lung development GO:0030324 IEA
    positive regulation of angiogenesis GO:0045766 IEA
    venous blood vessel morphogenesis GO:0048845 ISS
    positive regulation of vascular endothelial growth factor signaling pathway GO:1900748 IDA
    positive regulation of lymphangiogenesis GO:1901492 IEA
    lymphatic endothelial cell migration GO:1904977 IEA
Subcellular Localization
    collagen trimer GO:0005581 IEA
    extracellular space GO:0005615 IDA
    extracellular matrix GO:0031012 IDA
 Experiment description of studies that identified CCBE1 in sEVs
1
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CCBE1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRT27 342574
Two-hybrid Homo sapiens
2 POTEF 728378
Affinity Capture-MS Homo sapiens
3 FKBP15 23307
Affinity Capture-MS Homo sapiens
4 SLC25A31 83447
Affinity Capture-MS Homo sapiens
5 INCA1  
Two-hybrid Homo sapiens
6 VASP 7408
Affinity Capture-MS Homo sapiens
7 CEP55 55165
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 FBXO28  
Affinity Capture-MS Homo sapiens
9 KLHL12  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 KRT36 8689
Two-hybrid Homo sapiens
11 SIAH2 6478
Two-hybrid Homo sapiens
12 COPS6 10980
Two-hybrid Homo sapiens
13 TOX4  
Two-hybrid Homo sapiens
14 STX11 8676
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which CCBE1 is involved
No pathways found





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