Gene description for NEK10
Gene name NIMA-related kinase 10
Gene symbol NEK10
Other names/aliases -
Species Homo sapiens
 Database cross references - NEK10
ExoCarta ExoCarta_152110
Vesiclepedia VP_152110
Entrez Gene 152110
HGNC 18592
UniProt Q6ZWH5  
 NEK10 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Squamous carcinoma cells 20124223    
 Gene ontology annotations for NEK10
Molecular Function
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    metal ion binding GO:0046872 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    positive regulation of protein autophosphorylation GO:0031954 IDA
    positive regulation of MAP kinase activity GO:0043406 IMP
    regulation of ERK1 and ERK2 cascade GO:0070372 IMP
    mucociliary clearance GO:0120197 IMP
    regulation of cell cycle G2/M phase transition GO:1902749 IBA
    regulation of cell cycle G2/M phase transition GO:1902749 IMP
Subcellular Localization
    extracellular region GO:0005576 IEA
    protein kinase complex GO:1902911 IDA
 Experiment description of studies that identified NEK10 in sEVs
1
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 191
MISEV standards
Biophysical techniques
Alix|CD81|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NEK10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MYO9A 4649
Affinity Capture-MS Homo sapiens
2 KIAA0586  
Affinity Capture-MS Homo sapiens
3 HSPB1 3315
Two-hybrid Homo sapiens
4 MAP3K1 4214
Biochemical Activity Homo sapiens
5 EFNA4  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NEK10 is involved
No pathways found





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