Gene description for RDH10
Gene name retinol dehydrogenase 10 (all-trans)
Gene symbol RDH10
Other names/aliases SDR16C4
Species Homo sapiens
 Database cross references - RDH10
ExoCarta ExoCarta_157506
Vesiclepedia VP_157506
Entrez Gene 157506
HGNC 19975
MIM 607599
UniProt Q8IZV5  
 RDH10 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for RDH10
Molecular Function
    all-trans-retinol dehydrogenase (NAD+) activity GO:0004745 IDA
    all-trans-retinol dehydrogenase (NAD+) activity GO:0004745 TAS
    protein binding GO:0005515 IPI
    oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor GO:0016616 IBA
    all-trans-retinol dehydrogenase (NADP+) activity GO:0052650 TAS
Biological Process
    retinoid metabolic process GO:0001523 TAS
    metanephros development GO:0001656 IEA
    in utero embryonic development GO:0001701 IEA
    retinoic acid biosynthetic process GO:0002138 IEA
    visual perception GO:0007601 IDA
    gonad development GO:0008406 IEA
    neural crest cell development GO:0014032 IEA
    embryonic camera-type eye development GO:0031076 IEA
    embryonic forelimb morphogenesis GO:0035115 IEA
    retinol metabolic process GO:0042572 IDA
    retinol metabolic process GO:0042572 IEA
    retinal metabolic process GO:0042574 IDA
    ear development GO:0043583 IEA
    nose development GO:0043584 IEA
    embryonic viscerocranium morphogenesis GO:0048703 IEA
    primary lung bud formation GO:0060431 IEA
    bud elongation involved in lung branching GO:0060449 IEA
    positive regulation of retinoic acid biosynthetic process GO:1900054 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    lipid droplet GO:0005811 IBA
    membrane GO:0016020 IDA
 Experiment description of studies that identified RDH10 in sEVs
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for RDH10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ANLN 54443
Affinity Capture-MS Homo sapiens
2 GAD2  
Two-hybrid Homo sapiens
3 RMDN2 151393
Two-hybrid Homo sapiens
4 RAB39A 54734
Affinity Capture-MS Homo sapiens
5 ACSF2 80221
Two-hybrid Homo sapiens
6 DDA1  
Two-hybrid Homo sapiens
7 PIGH  
Affinity Capture-MS Homo sapiens
8 RPA3 6119
Proximity Label-MS Homo sapiens
9 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
10 GP9 2815
Affinity Capture-MS Homo sapiens
11 MORC3 23515
Affinity Capture-MS Homo sapiens
12 ERGIC1 57222
Affinity Capture-MS Homo sapiens
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