Gene description for Itga5
Gene name integrin alpha 5 (fibronectin receptor alpha)
Gene symbol Itga5
Other names/aliases Cd49e
Fnra
VLA5
Species Mus musculus
 Database cross references - Itga5
ExoCarta ExoCarta_16402
Vesiclepedia VP_16402
Entrez Gene 16402
UniProt P11688  
 Itga5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Itga5
Molecular Function
    epidermal growth factor receptor binding GO:0005154 IEA
    epidermal growth factor receptor binding GO:0005154 ISO
    integrin binding GO:0005178 IBA
    integrin binding GO:0005178 IPI
    integrin binding GO:0005178 ISO
    calcium ion binding GO:0005509 IEA
    calcium ion binding GO:0005509 ISO
    protein binding GO:0005515 IPI
    signaling receptor activity GO:0038023 IDA
    cell adhesion molecule binding GO:0050839 ISO
Biological Process
    angiogenesis GO:0001525 IBA
    cell-substrate junction assembly GO:0007044 IDA
    cell adhesion GO:0007155 ISO
    heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules GO:0007157 IMP
    leukocyte cell-cell adhesion GO:0007159 IMP
    cell-matrix adhesion GO:0007160 IBA
    integrin-mediated signaling pathway GO:0007229 IBA
    integrin-mediated signaling pathway GO:0007229 IEA
    integrin-mediated signaling pathway GO:0007229 ISO
    female pregnancy GO:0007565 IEA
    memory GO:0007613 IMP
    positive regulation of cell-substrate adhesion GO:0010811 IEA
    positive regulation of cell-substrate adhesion GO:0010811 ISO
    response to muscle activity GO:0014850 IDA
    CD40 signaling pathway GO:0023035 IEA
    CD40 signaling pathway GO:0023035 ISO
    positive regulation of cell migration GO:0030335 IEA
    positive regulation of cell migration GO:0030335 ISO
    cell-substrate adhesion GO:0031589 IEA
    cell-substrate adhesion GO:0031589 ISO
    cell adhesion mediated by integrin GO:0033627 IBA
    cell adhesion mediated by integrin GO:0033627 ISO
    cell adhesion mediated by integrin GO:0033627 ISS
    cell-cell adhesion mediated by integrin GO:0033631 IMP
    heterotypic cell-cell adhesion GO:0034113 IEA
    heterotypic cell-cell adhesion GO:0034113 ISO
    wound healing, spreading of epidermal cells GO:0035313 IEA
    endodermal cell differentiation GO:0035987 IEA
    endodermal cell differentiation GO:0035987 ISO
    regulation of angiogenesis GO:0045765 IGI
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IEA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    cell-cell adhesion GO:0098609 IBA
    positive regulation of sprouting angiogenesis GO:1903672 IEA
    positive regulation of sprouting angiogenesis GO:1903672 ISO
    negative regulation of anoikis GO:2000811 IEA
    negative regulation of anoikis GO:2000811 ISO
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    cell-cell junction GO:0005911 IEA
    cell-cell junction GO:0005911 ISO
    focal adhesion GO:0005925 ISO
    focal adhesion GO:0005925 ISO
    focal adhesion GO:0005925 ISS
    integrin complex GO:0008305 IBA
    integrin complex GO:0008305 ISO
    external side of plasma membrane GO:0009897 IBA
    external side of plasma membrane GO:0009897 IDA
    cell surface GO:0009986 IDA
    cell surface GO:0009986 ISO
    cell surface GO:0009986 ISS
    cytoplasmic vesicle GO:0031410 IDA
    ruffle membrane GO:0032587 IEA
    ruffle membrane GO:0032587 ISO
    integrin alpha5-beta1 complex GO:0034674 IEA
    integrin alpha5-beta1 complex GO:0034674 ISO
    synapse GO:0045202 IDA
    postsynaptic membrane GO:0045211 IEA
    postsynaptic membrane GO:0045211 ISO
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Itga5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Itga5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TPRN 286262
Affinity Capture-MS Homo sapiens
2 ITGB1 3688
Affinity Capture-MS Homo sapiens
3 ACTR2 10097
Affinity Capture-MS Homo sapiens
4 RBBP6 5930
Affinity Capture-MS Homo sapiens
5 TMOD3 29766
Affinity Capture-MS Homo sapiens
6 OGT 8473
Affinity Capture-MS Homo sapiens
7 AP2B1 163
Affinity Capture-MS Homo sapiens
8 PPP1CB 5500
Affinity Capture-MS Homo sapiens
9 ARPC5L 81873
Affinity Capture-MS Homo sapiens
10 ARPC1B 10095
Affinity Capture-MS Homo sapiens
11 TPM2 7169
Affinity Capture-MS Homo sapiens
12 Sharpin  
FRET Mus musculus
13 Rtn4 68585
Affinity Capture-Western Mus musculus
14 LIMCH1 22998
Affinity Capture-MS Homo sapiens
15 SPECC1 92521
Affinity Capture-MS Homo sapiens
16 MYO5A 4644
Affinity Capture-MS Homo sapiens
17 LUZP1 7798
Affinity Capture-MS Homo sapiens
18 TMOD1  
Affinity Capture-MS Homo sapiens
19 Atxn3  
Affinity Capture-Western Mus musculus
20 Tmem173  
Proximity Label-MS Mus musculus
21 CAPZB 832
Affinity Capture-MS Homo sapiens
22 LRRFIP2 9209
Affinity Capture-MS Homo sapiens
23 TPM1 7168
Affinity Capture-MS Homo sapiens
24 MYO6 4646
Affinity Capture-MS Homo sapiens
25 MICAL3 57553
Affinity Capture-MS Homo sapiens
26 DAPK3 1613
Affinity Capture-MS Homo sapiens
27 FLNA 2316
Affinity Capture-MS Homo sapiens
28 ACTR3 10096
Affinity Capture-MS Homo sapiens
29 SPECC1L 23384
Affinity Capture-MS Homo sapiens
30 ARPC5 10092
Affinity Capture-MS Homo sapiens
31 MYO18A 399687
Affinity Capture-MS Homo sapiens
32 TPM3 7170
Affinity Capture-MS Homo sapiens
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