Gene description for Lrp1
Gene name low density lipoprotein receptor-related protein 1
Gene symbol Lrp1
Other names/aliases A2mr
AI316852
CD91
Lrp
b2b1554Clo
Species Mus musculus
 Database cross references - Lrp1
ExoCarta ExoCarta_16971
Vesiclepedia VP_16971
Entrez Gene 16971
UniProt Q91ZX7  
 Lrp1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Lrp1
Molecular Function
    protease binding GO:0002020 ISO
    signaling receptor binding GO:0005102 IPI
    calcium ion binding GO:0005509 IDA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    coreceptor activity GO:0015026 ISO
    alpha-2 macroglobulin receptor activity GO:0016964 IEA
    alpha-2 macroglobulin receptor activity GO:0016964 ISO
    alpha-2 macroglobulin receptor activity GO:0016964 ISS
    clathrin heavy chain binding GO:0032050 IEA
    clathrin heavy chain binding GO:0032050 ISO
    apolipoprotein binding GO:0034185 IBA
    apolipoprotein binding GO:0034185 IEA
    apolipoprotein binding GO:0034185 ISO
    cargo receptor activity GO:0038024 IBA
    cargo receptor activity GO:0038024 IMP
    cargo receptor activity GO:0038024 ISO
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
Biological Process
    astrocyte activation involved in immune response GO:0002265 IGI
    cardiac septum development GO:0003279 IMP
    receptor-mediated endocytosis GO:0006898 IBA
    receptor-mediated endocytosis GO:0006898 IDA
    receptor-mediated endocytosis GO:0006898 IEA
    receptor-mediated endocytosis GO:0006898 IGI
    receptor-mediated endocytosis GO:0006898 IMP
    receptor-mediated endocytosis GO:0006898 ISO
    phagocytosis GO:0006909 IBA
    phagocytosis GO:0006909 IEA
    phagocytosis GO:0006909 ISO
    lysosomal transport GO:0007041 IDA
    enzyme-linked receptor protein signaling pathway GO:0007167 IMP
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 ISO
    positive regulation of cytosolic calcium ion concentration GO:0007204 ISO
    cholesterol metabolic process GO:0008203 IMP
    negative regulation of gene expression GO:0010629 IMP
    negative regulation of cell-substrate adhesion GO:0010812 ISO
    positive regulation of cholesterol efflux GO:0010875 IMP
    positive regulation of neuron projection development GO:0010976 ISO
    negative regulation of neuron projection development GO:0010977 ISO
    negative regulation of smooth muscle cell migration GO:0014912 IMP
    lipid catabolic process GO:0016042 ISO
    negative regulation of Wnt signaling pathway GO:0030178 IDA
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IGI
    positive regulation of protein binding GO:0032092 ISO
    positive regulation of lipid transport GO:0032370 IMP
    regulation of cholesterol transport GO:0032374 IMP
    regulation of phospholipase A2 activity GO:0032429 IMP
    regulation of actin cytoskeleton organization GO:0032956 IMP
    positive regulation of insulin secretion involved in cellular response to glucose stimulus GO:0035774 ISO
    aorta morphogenesis GO:0035909 IMP
    negative regulation of apoptotic process GO:0043066 ISO
    apoptotic cell clearance GO:0043277 IMP
    negative regulation of neuron apoptotic process GO:0043524 ISO
    positive regulation of axon extension GO:0045773 ISO
    positive regulation of endocytosis GO:0045807 IEA
    positive regulation of endocytosis GO:0045807 ISO
    positive regulation of axon extension involved in regeneration GO:0048691 ISO
    positive regulation of collateral sprouting of injured axon GO:0048694 ISO
    positive regulation of phagocytosis GO:0050766 ISO
    positive regulation of protein transport GO:0051222 ISO
    negative regulation of cytosolic calcium ion concentration GO:0051481 ISO
    negative regulation of focal adhesion assembly GO:0051895 ISO
    negative regulation of SMAD protein signal transduction GO:0060392 IMP
    coronary vasculature development GO:0060976 IMP
    chemoattraction of axon GO:0061642 ISO
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    amyloid-beta clearance GO:0097242 IMP
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 EXP
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 IDA
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 IMP
    amyloid-beta clearance by transcytosis GO:0150093 IBA
    amyloid-beta clearance by transcytosis GO:0150093 IDA
    amyloid-beta clearance by transcytosis GO:0150093 IEA
    amyloid-beta clearance by transcytosis GO:0150093 ISO
    amyloid-beta clearance by cellular catabolic process GO:0150094 IBA
    amyloid-beta clearance by cellular catabolic process GO:0150094 IDA
    amyloid-beta clearance by cellular catabolic process GO:0150094 IEA
    amyloid-beta clearance by cellular catabolic process GO:0150094 IGI
    amyloid-beta clearance by cellular catabolic process GO:0150094 IMP
    amyloid-beta clearance by cellular catabolic process GO:0150094 ISO
    transport across blood-brain barrier GO:0150104 IMP
    positive regulation of Schwann cell migration GO:1900149 ISO
    positive regulation of amyloid-beta clearance GO:1900223 IMP
    positive regulation of protein localization to plasma membrane GO:1903078 IEA
    positive regulation of protein localization to plasma membrane GO:1903078 ISO
    positive regulation of cholesterol import GO:1904109 ISO
    positive regulation of transcytosis GO:1904300 IMP
    cellular response to amyloid-beta GO:1904646 IGI
    positive regulation of vascular associated smooth muscle cell migration GO:1904754 ISO
    regulation of pulmonary blood vessel remodeling GO:1905109 IMP
    positive regulation of lysosomal protein catabolic process GO:1905167 IEA
    positive regulation of lysosomal protein catabolic process GO:1905167 ISO
    positive regulation of chemokine (C-X-C motif) ligand 2 production GO:2000343 ISO
    negative regulation of platelet-derived growth factor receptor-beta signaling pathway GO:2000587 IMP
Subcellular Localization
    nucleus GO:0005634 IEA
    endosome GO:0005768 ISO
    early endosome GO:0005769 IEA
    early endosome GO:0005769 ISO
    Golgi apparatus GO:0005794 IEA
    microtubule organizing center GO:0005815 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    clathrin-coated pit GO:0005905 IEA
    external side of plasma membrane GO:0009897 IDA
    membrane GO:0016020 IDA
    basolateral plasma membrane GO:0016323 IEA
    basolateral plasma membrane GO:0016323 ISO
    clathrin-coated vesicle GO:0030136 ISO
    dendrite GO:0030425 ISO
    insulin-responsive compartment GO:0032593 ISO
    neuronal cell body GO:0043025 ISO
    receptor complex GO:0043235 IEA
    receptor complex GO:0043235 ISO
    axonal growth cone GO:0044295 ISO
    apical part of cell GO:0045177 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    postsynaptic density membrane GO:0098839 IDA
    postsynaptic density membrane GO:0098839 IEP
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IEP
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Lrp1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Lrp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PID1 55022
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
2 Tmem173  
Proximity Label-MS Mus musculus
3 Grin2b  
Co-fractionation Mus musculus
4 Tgfbr1 21812
Affinity Capture-Western Mus musculus
5 Gpr98  
Co-fractionation Mus musculus
6 Dlg4  
Co-fractionation Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
7 Aspscr1  
Affinity Capture-Western Mus musculus
8 Fmr1  
Affinity Capture-MS Mus musculus
9 Gria1  
Co-fractionation Mus musculus
10 Grin2a  
Co-fractionation Mus musculus
11 Ntrk2  
Co-fractionation Mus musculus
12 Ranbp9  
Affinity Capture-Western Mus musculus
13 Lifr  
Co-fractionation Mus musculus
14 Fancd2  
Affinity Capture-MS Mus musculus
15 Grin1  
Co-fractionation Mus musculus
16 Mttp  
Co-fractionation Mus musculus
17 Ptprn2  
Co-fractionation Mus musculus
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