Gene description for Pabpc1
Gene name poly(A) binding protein, cytoplasmic 1
Gene symbol Pabpc1
Other names/aliases Pabp
Pabp1
Species Rattus norvegicus
 Database cross references - Pabpc1
ExoCarta ExoCarta_171350
Vesiclepedia VP_171350
Entrez Gene 171350
UniProt Q9EPH8  
 Pabpc1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Pabpc1
Molecular Function
    RNA binding GO:0003723 IEA
    RNA binding GO:0003723 ISO
    mRNA binding GO:0003729 IDA
    mRNA binding GO:0003729 ISO
    mRNA 3'-UTR binding GO:0003730 IBA
    mRNA 3'-UTR binding GO:0003730 ISO
    protein binding GO:0005515 IPI
    poly(A) binding GO:0008143 IBA
    poly(A) binding GO:0008143 ISO
    poly(A) binding GO:0008143 ISS
    poly(U) RNA binding GO:0008266 IBA
    poly(U) RNA binding GO:0008266 ISO
Biological Process
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 IEA
    mRNA processing GO:0006397 IEA
    biological_process GO:0008150 ND
    RNA splicing GO:0008380 IEA
    regulatory ncRNA-mediated gene silencing GO:0031047 ISO
    regulatory ncRNA-mediated gene silencing GO:0031047 ISS
    positive regulation of viral genome replication GO:0045070 ISO
    positive regulation of nuclear-transcribed mRNA poly(A) tail shortening GO:0060213 ISO
    positive regulation of nuclear-transcribed mRNA poly(A) tail shortening GO:0060213 ISS
    CRD-mediated mRNA stabilization GO:0070934 ISO
    negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:1900152 ISO
    positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:1900153 ISO
    positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:1900153 ISS
    negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:2000623 ISO
    negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:2000623 ISS
    positive regulation of cytoplasmic translation GO:2000767 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytoplasmic stress granule GO:0010494 IBA
    cytoplasmic stress granule GO:0010494 ISO
    cytoplasmic stress granule GO:0010494 ISS
    lamellipodium GO:0030027 IEA
    dendrite GO:0030425 IDA
    cell leading edge GO:0031252 ISO
    cell leading edge GO:0031252 ISS
    protein-containing complex GO:0032991 IDA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    synapse GO:0045202 ISO
    catalytic step 2 spliceosome GO:0071013 ISO
    mCRD-mediated mRNA stability complex GO:0106002 ISO
    ribonucleoprotein complex GO:1990904 IBA
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Pabpc1 in sEVs
1
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pabpc1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STAU1 6780
Reconstituted Complex Homo sapiens
2 Grb7  
Affinity Capture-MS Rattus norvegicus
3 Slc8a1  
Reconstituted Complex Mus musculus
4 Dao  
Affinity Capture-MS Rattus norvegicus
5 Tob2  
Affinity Capture-Western Rattus norvegicus
6 Slc8a1  
Affinity Capture-MS Rattus norvegicus
7 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
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