Gene description for Man2b1
Gene name mannosidase 2, alpha B1
Gene symbol Man2b1
Other names/aliases AW107687
LAMAN
Species Mus musculus
 Database cross references - Man2b1
ExoCarta ExoCarta_17159
Vesiclepedia VP_17159
Entrez Gene 17159
UniProt O09159  
 Man2b1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Man2b1
Molecular Function
    alpha-mannosidase activity GO:0004559 IBA
    alpha-mannosidase activity GO:0004559 IDA
    alpha-mannosidase activity GO:0004559 IMP
    alpha-mannosidase activity GO:0004559 ISO
    D-mannose binding GO:0005537 IEA
    D-mannose binding GO:0005537 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    mannose metabolic process GO:0006013 IDA
    mannose metabolic process GO:0006013 IMP
    learning or memory GO:0007611 IMP
    oligosaccharide catabolic process GO:0009313 IDA
    protein modification process GO:0036211 IEA
    protein modification process GO:0036211 ISO
Subcellular Localization
    extracellular space GO:0005615 IEA
    extracellular space GO:0005615 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    lysosome GO:0005764 IBA
    lysosomal lumen GO:0043202 IDA
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Man2b1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Man2b1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmx3  
Co-fractionation Mus musculus
2 Adgre5 26364
Co-fractionation Mus musculus
3 Dnase2a  
Co-fractionation Mus musculus
4 Siae  
Co-fractionation Mus musculus
5 P4htm  
Co-fractionation Mus musculus
6 Sumf1  
Co-fractionation Mus musculus
7 Fut11  
Co-fractionation Mus musculus
View the network image/svg+xml



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