Gene description for Nid1
Gene name nidogen 1
Gene symbol Nid1
Other names/aliases A630025O17
Nid
entactin
entactin-1
nidogen-1
Species Mus musculus
 Database cross references - Nid1
ExoCarta ExoCarta_18073
Vesiclepedia VP_18073
Entrez Gene 18073
UniProt P10493  
 Nid1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nid1
Molecular Function
    extracellular matrix structural constituent GO:0005201 RCA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    collagen binding GO:0005518 IDA
    collagen binding GO:0005518 ISO
    laminin binding GO:0043236 ISO
    laminin-1 binding GO:0043237 IDA
    proteoglycan binding GO:0043394 IEA
    proteoglycan binding GO:0043394 ISO
    extracellular matrix binding GO:0050840 IDA
Biological Process
    cell-matrix adhesion GO:0007160 IDA
    positive regulation of cell-substrate adhesion GO:0010811 IDA
    extracellular matrix organization GO:0030198 IDA
    glomerular basement membrane development GO:0032836 IMP
    positive regulation of cell adhesion GO:0045785 NAS
    positive regulation of muscle cell differentiation GO:0051149 NAS
    regulation of basement membrane organization GO:0110011 NAS
    positive regulation of integrin-mediated signaling pathway GO:2001046 NAS
Subcellular Localization
    extracellular region GO:0005576 TAS
    basement membrane GO:0005604 IDA
    basement membrane GO:0005604 IPI
    basement membrane GO:0005604 ISO
    basement membrane GO:0005604 NAS
    basement membrane GO:0005604 TAS
    extracellular matrix GO:0031012 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
    cell periphery GO:0071944 IDA
    protein complex involved in cell-matrix adhesion GO:0098637 NAS
 Experiment description of studies that identified Nid1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nid1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COL1A1 1277
Reconstituted Complex Homo sapiens
2 Hspg2 15530
Co-fractionation Mus musculus
3 LAMC1 3915
Reconstituted Complex Homo sapiens
4 Eed  
Affinity Capture-MS Mus musculus
5 Lama1  
Co-fractionation Mus musculus
6 COL4A1 1282
Reconstituted Complex Homo sapiens
7 Fbln1 14114
Co-fractionation Mus musculus
8 Hspb2 69253
Affinity Capture-MS Mus musculus
9 Fga 14161
Co-fractionation Mus musculus
10 Fgb  
Co-fractionation Mus musculus
11 Col4a3  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Nid1 is involved
PathwayEvidenceSource
Degradation of the extracellular matrix IEA Reactome
Extracellular matrix organization IEA Reactome
Laminin interactions IEA Reactome





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