Gene description for Pafah1b2
Gene name platelet-activating factor acetylhydrolase, isoform 1b, subunit 2
Gene symbol Pafah1b2
Other names/aliases AI747451
AU021353
Pafahb
mus[b]
Species Mus musculus
 Database cross references - Pafah1b2
ExoCarta ExoCarta_18475
Vesiclepedia VP_18475
Entrez Gene 18475
UniProt Q61206  
 Pafah1b2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pafah1b2
Molecular Function
    1-alkyl-2-acetylglycerophosphocholine esterase activity GO:0003847 ISO
    1-alkyl-2-acetylglycerophosphocholine esterase activity GO:0003847 ISS
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISS
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    protein heterodimerization activity GO:0046982 ISS
    platelet-activating factor acetyltransferase activity GO:0047179 IBA
    platelet-activating factor acetyltransferase activity GO:0047179 IEA
    platelet-activating factor acetyltransferase activity GO:0047179 ISO
Biological Process
    spermatogenesis GO:0007283 IGI
    spermatogenesis GO:0007283 IMP
    spermatogenesis GO:0007283 IMP
    lipid catabolic process GO:0016042 IEA
    positive regulation of macroautophagy GO:0016239 IEA
    positive regulation of macroautophagy GO:0016239 ISO
Subcellular Localization
    fibrillar center GO:0001650 IEA
    fibrillar center GO:0001650 ISO
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    1-alkyl-2-acetylglycerophosphocholine esterase complex GO:0008247 ISO
    1-alkyl-2-acetylglycerophosphocholine esterase complex GO:0008247 ISS
 Experiment description of studies that identified Pafah1b2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pafah1b2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Vldlr  
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 PAFAH1B1 5048
Affinity Capture-MS Homo sapiens
4 PAFAH1B3 5050
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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