Gene description for Pecam1
Gene name platelet/endothelial cell adhesion molecule 1
Gene symbol Pecam1
Other names/aliases C85791
Cd31
PECAM-1
Pecam
Species Mus musculus
 Database cross references - Pecam1
ExoCarta ExoCarta_18613
Vesiclepedia VP_18613
Entrez Gene 18613
UniProt Q08481  
 Pecam1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pecam1
Molecular Function
    transmembrane signaling receptor activity GO:0004888 IBA
    protein binding GO:0005515 IPI
    protein phosphatase binding GO:0019903 ISO
    protein homodimerization activity GO:0042803 ISO
Biological Process
    angiogenesis GO:0001525 IMP
    endothelial cell morphogenesis GO:0001886 IMP
    positive regulation of protein phosphorylation GO:0001934 ISO
    positive regulation of leukocyte migration GO:0002687 ISO
    positive regulation of cellular extravasation GO:0002693 ISO
    phagocytosis GO:0006909 ISO
    immune response GO:0006955 IBA
    cell adhesion GO:0007155 IDA
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 ISO
    leukocyte cell-cell adhesion GO:0007159 ISO
    cell surface receptor signaling pathway GO:0007166 IBA
    Rho protein signal transduction GO:0007266 IMP
    regulation of cell migration GO:0030334 IMP
    positive regulation of cell migration GO:0030335 ISO
    negative regulation of actin filament polymerization GO:0030837 ISO
    monocyte extravasation GO:0035696 ISO
    wound healing GO:0042060 IMP
    positive regulation of tyrosine phosphorylation of STAT protein GO:0042531 IDA
    endothelial cell migration GO:0043542 IMP
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    negative regulation of peptidyl-tyrosine phosphorylation GO:0050732 ISO
    diapedesis GO:0050904 ISO
    establishment of endothelial barrier GO:0061028 ISO
    bicellular tight junction assembly GO:0070830 ISO
    neutrophil extravasation GO:0072672 ISO
    endothelial cell-matrix adhesion GO:0090673 IMP
    cell-cell adhesion GO:0098609 ISO
    cell-cell adhesion via plasma-membrane adhesion molecules GO:0098742 IBA
    cell-cell adhesion via plasma-membrane adhesion molecules GO:0098742 ISO
    positive regulation of protein localization to cell-cell junction GO:0150107 ISO
Subcellular Localization
    ruffle GO:0001726 ISO
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    cell-cell junction GO:0005911 ISO
    external side of plasma membrane GO:0009897 IBA
    external side of plasma membrane GO:0009897 IDA
    cell surface GO:0009986 ISO
    membrane GO:0016020 IDA
    smooth muscle contractile fiber GO:0030485 IDA
    protein-containing complex GO:0032991 ISO
    cell-cell contact zone GO:0044291 IDA
    membrane raft GO:0045121 IDA
    cell periphery GO:0071944 IDA
 Experiment description of studies that identified Pecam1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pecam1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hck  
Co-fractionation Mus musculus
2 Gpc1 14733
Co-fractionation Mus musculus
3 Fam120b 67544
Co-fractionation Mus musculus
4 Inpp5d 16331
Co-fractionation Mus musculus
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