Gene description for Pfkl
Gene name phosphofructokinase, liver, B-type
Gene symbol Pfkl
Other names/aliases AA407869
ATP-PFK
PFK-L
Species Mus musculus
 Database cross references - Pfkl
ExoCarta ExoCarta_18641
Vesiclepedia VP_18641
Entrez Gene 18641
UniProt P12382  
 Pfkl identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pfkl
Molecular Function
    6-phosphofructokinase activity GO:0003872 IDA
    6-phosphofructokinase activity GO:0003872 ISO
    6-phosphofructokinase activity GO:0003872 ISO
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    kinase binding GO:0019900 IEA
    kinase binding GO:0019900 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    metal ion binding GO:0046872 IEA
    monosaccharide binding GO:0048029 ISO
    fructose binding GO:0070061 IEA
    fructose binding GO:0070061 ISO
    fructose-6-phosphate binding GO:0070095 IBA
    fructose-6-phosphate binding GO:0070095 IEA
    fructose-6-phosphate binding GO:0070095 ISO
Biological Process
    fructose 6-phosphate metabolic process GO:0006002 IBA
    fructose 6-phosphate metabolic process GO:0006002 IDA
    fructose 6-phosphate metabolic process GO:0006002 ISO
    glycolytic process GO:0006096 ISO
    response to glucose GO:0009749 IDA
    response to glucose GO:0009749 ISO
    fructose 1,6-bisphosphate metabolic process GO:0030388 IBA
    fructose 1,6-bisphosphate metabolic process GO:0030388 ISO
    fructose 1,6-bisphosphate metabolic process GO:0030388 ISS
    negative regulation of insulin secretion GO:0046676 IDA
    glycolytic process through fructose-6-phosphate GO:0061615 IDA
    glycolytic process through fructose-6-phosphate GO:0061615 IDA
    glycolytic process through fructose-6-phosphate GO:0061615 IMP
    canonical glycolysis GO:0061621 IBA
Subcellular Localization
    cytosol GO:0005829 IDA
    6-phosphofructokinase complex GO:0005945 IBA
    6-phosphofructokinase complex GO:0005945 IEA
    6-phosphofructokinase complex GO:0005945 ISO
    membrane GO:0016020 IBA
 Experiment description of studies that identified Pfkl in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pfkl
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sfpq 71514
Co-fractionation Mus musculus
2 Lnx1  
Affinity Capture-MS Mus musculus
3 Atxn1  
Proximity Label-MS Mus musculus
4 Foxp3  
Affinity Capture-MS Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Mapt  
Affinity Capture-MS Mus musculus
7 Tubb5 22154
Co-fractionation Mus musculus
8 Fancd2  
Affinity Capture-MS Mus musculus
9 Grin1  
Affinity Capture-MS Mus musculus
10 Ywhaz 22631
Affinity Capture-MS Mus musculus
11 Kctd13  
Affinity Capture-MS Mus musculus
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