Gene description for Pnp
Gene name purine-nucleoside phosphorylase
Gene symbol Pnp
Other names/aliases AL024301
AU015798
Np
Np-1
Np-2
Pnp1
Species Mus musculus
 Database cross references - Pnp
ExoCarta ExoCarta_18950
Vesiclepedia VP_18950
Entrez Gene 18950
UniProt P23492  
 Pnp identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pnp
Molecular Function
    nucleoside binding GO:0001882 ISO
    purine nucleobase binding GO:0002060 ISO
    purine-nucleoside phosphorylase activity GO:0004731 IBA
    purine-nucleoside phosphorylase activity GO:0004731 IDA
    purine-nucleoside phosphorylase activity GO:0004731 IMP
    purine-nucleoside phosphorylase activity GO:0004731 ISO
    phosphate ion binding GO:0042301 ISO
    identical protein binding GO:0042802 ISO
    guanosine phosphorylase activity GO:0047975 IDA
Biological Process
    allantoin metabolic process GO:0000255 IDA
    allantoin metabolic process GO:0000255 IMP
    allantoin metabolic process GO:0000255 ISO
    positive regulation of T cell mediated cytotoxicity GO:0001916 IMP
    nucleobase-containing compound metabolic process GO:0006139 ISO
    inosine catabolic process GO:0006148 IDA
    inosine catabolic process GO:0006148 IMP
    inosine catabolic process GO:0006148 IMP
    inosine catabolic process GO:0006148 ISO
    inosine catabolic process GO:0006148 ISO
    deoxyinosine catabolic process GO:0006149 IMP
    deoxyinosine catabolic process GO:0006149 IMP
    deoxyinosine catabolic process GO:0006149 ISO
    adenosine catabolic process GO:0006154 IDA
    adenosine catabolic process GO:0006154 IMP
    deoxyadenosine catabolic process GO:0006157 IMP
    deoxyadenosine catabolic process GO:0006157 ISO
    deoxyguanosine catabolic process GO:0006161 IMP
    deoxyguanosine catabolic process GO:0006161 IMP
    purine ribonucleoside salvage GO:0006166 IEA
    GTP biosynthetic process GO:0006183 IMP
    AMP catabolic process GO:0006196 IDA
    AMP catabolic process GO:0006196 IMP
    GMP catabolic process to guanine GO:0006202 IMP
    IMP catabolic process GO:0006204 IMP
    IMP catabolic process GO:0006204 ISO
    DNA repair GO:0006281 IMP
    nicotinamide riboside catabolic process GO:0006738 ISO
    immune response GO:0006955 ISO
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IMP
    apoptotic mitochondrial changes GO:0008637 IMP
    nucleotide biosynthetic process GO:0009165 ISO
    response to xenobiotic stimulus GO:0009410 ISO
    response to gamma radiation GO:0010332 IMP
    B cell differentiation GO:0030183 IMP
    GMP salvage GO:0032263 IDA
    IMP salvage GO:0032264 IDA
    positive regulation of interleukin-2 production GO:0032743 ISO
    urate biosynthetic process GO:0034418 IMP
    urate biosynthetic process GO:0034418 ISO
    T cell proliferation GO:0042098 IMP
    positive regulation of T cell proliferation GO:0042102 IMP
    positive regulation of T cell proliferation GO:0042102 ISO
    purine nucleoside metabolic process GO:0042278 IC
    purine nucleoside metabolic process GO:0042278 IDA
    purine nucleoside metabolic process GO:0042278 IMP
    purine-containing compound salvage GO:0043101 ISO
    amide catabolic process GO:0043605 IDA
    amide catabolic process GO:0043605 IMP
    amide catabolic process GO:0043605 ISO
    AMP salvage GO:0044209 IDA
    positive regulation of B cell differentiation GO:0045579 IMP
    positive regulation of DNA repair GO:0045739 IMP
    GMP catabolic process GO:0046038 IMP
    dGMP catabolic process GO:0046055 IMP
    dGMP catabolic process GO:0046055 ISO
    dAMP catabolic process GO:0046059 IMP
    dAMP catabolic process GO:0046059 ISO
    dGTP metabolic process GO:0046070 IMP
    guanosine catabolic process GO:0046115 IMP
    alpha-beta T cell differentiation GO:0046632 IMP
    positive regulation of alpha-beta T cell differentiation GO:0046638 IMP
    positive regulation of alpha-beta T cell differentiation GO:0046638 ISO
    T cell apoptotic process GO:0070231 IMP
    negative regulation of T cell apoptotic process GO:0070233 IMP
Subcellular Localization
    extracellular region GO:0005576 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Pnp in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pnp
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tfam  
Co-fractionation Mus musculus
2 Fancd2  
Affinity Capture-MS Mus musculus
3 Psmd5 66998
Co-fractionation Mus musculus
4 Uchl5 56207
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here