Gene description for Pstpip1
Gene name proline-serine-threonine phosphatase-interacting protein 1
Gene symbol Pstpip1
Other names/aliases CD2BP1
def-2
Species Mus musculus
 Database cross references - Pstpip1
ExoCarta ExoCarta_19200
Vesiclepedia VP_19200
Entrez Gene 19200
UniProt P97814  
 Pstpip1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pstpip1
Molecular Function
    actin binding GO:0003779 IDA
    protein binding GO:0005515 IPI
    protein phosphatase binding GO:0019903 IDA
    identical protein binding GO:0042802 ISO
Biological Process
    cytokinesis GO:0000910 IC
    endocytosis GO:0006897 IEA
    inflammatory response GO:0006954 IEA
    cell adhesion GO:0007155 IEA
    innate immune response GO:0045087 IEA
Subcellular Localization
    stress fiber GO:0001725 IDA
    uropod GO:0001931 IEA
    cytoplasm GO:0005737 IBA
    actomyosin contractile ring GO:0005826 IDA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    lamellipodium GO:0030027 IEA
    cleavage furrow GO:0032154 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified Pstpip1 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pstpip1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 WAS 7454
Two-hybrid Homo sapiens
2 Inpp5d 16331
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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