Gene description for Rab22a
Gene name RAB22A, member RAS oncogene family
Gene symbol Rab22a
Other names/aliases 3732413A17Rik
AI662177
AW319644
AW550514
E130120E14Rik
Rab22
Species Mus musculus
 Database cross references - Rab22a
ExoCarta ExoCarta_19334
Vesiclepedia VP_19334
Entrez Gene 19334
UniProt P35285  
 Rab22a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rab22a
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    GTPase activity GO:0003924 ISO
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IDA
    GTP binding GO:0005525 ISO
    GDP binding GO:0019003 ISO
    GDP binding GO:0019003 ISS
Biological Process
    intracellular protein transport GO:0006886 IBA
    endocytosis GO:0006897 IBA
    endocytosis GO:0006897 IEA
    endocytosis GO:0006897 ISO
    endosome organization GO:0007032 IEA
    protein transport GO:0015031 IEA
    regulation of vesicle size GO:0097494 IEA
    regulation of vesicle size GO:0097494 ISO
Subcellular Localization
    ruffle GO:0001726 IEA
    early endosome GO:0005769 IBA
    early endosome GO:0005769 IEA
    early endosome GO:0005769 ISO
    late endosome GO:0005770 IEA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    endosome membrane GO:0010008 IEA
    endomembrane system GO:0012505 IBA
    actin cytoskeleton GO:0015629 IDA
    phagocytic vesicle membrane GO:0030670 IEA
    phagocytic vesicle GO:0045335 ISO
    phagocytic vesicle GO:0045335 ISS
 Experiment description of studies that identified Rab22a in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
EM
Biophysical techniques
HSP70|HSP90
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rab22a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SMURF2 64750
Affinity Capture-Luminescence Homo sapiens
View the network image/svg+xml
 Pathways in which Rab22a is involved
PathwayEvidenceSource
Metabolism of proteins IEA Reactome
Post-translational protein modification IEA Reactome
RAB geranylgeranylation IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here