Gene description for Rab6a
Gene name RAB6A, member RAS oncogene family
Gene symbol Rab6a
Other names/aliases 2610028L11Rik
AA419671
Rab6
Species Mus musculus
 Database cross references - Rab6a
ExoCarta ExoCarta_19346
Vesiclepedia VP_19346
Entrez Gene 19346
UniProt P35279  
 Rab6a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rab6a
Molecular Function
    ATPase activator activity GO:0001671 ISO
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 ISS
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 ISO
    GTP binding GO:0005525 ISS
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    myosin V binding GO:0031489 IEA
    myosin V binding GO:0031489 ISO
    ATPase binding GO:0051117 ISO
Biological Process
    intracellular protein transport GO:0006886 IBA
    intracellular protein transport GO:0006886 TAS
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 IBA
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 ISO
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 ISS
    intra-Golgi vesicle-mediated transport GO:0006891 IBA
    peptidyl-cysteine methylation GO:0018125 ISO
    antigen processing and presentation GO:0019882 IEA
    antigen processing and presentation GO:0019882 ISO
    protein localization to Golgi apparatus GO:0034067 ISO
    protein localization to Golgi apparatus GO:0034067 ISS
    early endosome to Golgi transport GO:0034498 IEA
    early endosome to Golgi transport GO:0034498 ISO
    retrograde transport, endosome to Golgi GO:0042147 IBA
    protein localization to Golgi membrane GO:1903292 IBA
    protein localization to Golgi membrane GO:1903292 ISO
    protein localization to Golgi membrane GO:1903292 ISS
Subcellular Localization
    Golgi membrane GO:0000139 ISO
    Golgi membrane GO:0000139 ISS
    acrosomal membrane GO:0002080 IDA
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    trans-Golgi network GO:0005802 IEA
    trans-Golgi network GO:0005802 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    endomembrane system GO:0012505 IBA
    membrane GO:0016020 ISO
    cytoplasmic vesicle GO:0031410 ISO
    endosome to plasma membrane transport vesicle GO:0070381 IEA
    endosome to plasma membrane transport vesicle GO:0070381 ISO
    presynapse GO:0098793 ISO
 Experiment description of studies that identified Rab6a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rab6a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atxn1  
Affinity Capture-MS Mus musculus
2 EIF4ENIF1 56478
Two-hybrid Homo sapiens
3 GDI2 2665
Two-hybrid Homo sapiens
4 Ywhae 22627
Two-hybrid Mus musculus
5 Gdi2 14569
Two-hybrid Mus musculus
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