Gene description for Rad23b
Gene name RAD23b homolog (S. cerevisiae)
Gene symbol Rad23b
Other names/aliases 0610007D13Rik
AV001138
HR23B
mHR23B
p58
Species Mus musculus
 Database cross references - Rad23b
ExoCarta ExoCarta_19359
Vesiclepedia VP_19359
Entrez Gene 19359
UniProt P54728  
 Rad23b identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rad23b
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    damaged DNA binding GO:0003684 IEA
    protein binding GO:0005515 IPI
    polyubiquitin modification-dependent protein binding GO:0031593 IBA
    polyubiquitin modification-dependent protein binding GO:0031593 IEA
    polyubiquitin modification-dependent protein binding GO:0031593 ISO
    ubiquitin binding GO:0043130 IBA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IPI
    proteasome binding GO:0070628 IBA
    DNA damage sensor activity GO:0140612 IEA
Biological Process
    nucleotide-excision repair GO:0006289 IEA
    nucleotide-excision repair GO:0006289 ISO
    DNA damage response GO:0006974 IDA
    spermatogenesis GO:0007283 IMP
    regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032434 IEA
    regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032434 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IEA
    embryonic organ development GO:0048568 IEA
    cellular response to interleukin-7 GO:0098761 IDA
Subcellular Localization
    proteasome complex GO:0000502 IEA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    XPC complex GO:0071942 ISO
    XPC complex GO:0071942 ISS
 Experiment description of studies that identified Rad23b in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rad23b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ubqln2  
Co-fractionation Mus musculus
2 Psmd8 57296
Co-fractionation Mus musculus
3 Zfand2b  
Co-fractionation Mus musculus
4 Ubc  
Reconstituted Complex Mus musculus
5 Uba6 231380
Co-fractionation Mus musculus
6 Ubqln1  
Co-fractionation Mus musculus
7 Uba3  
Co-fractionation Mus musculus
8 Uba2 50995
Co-fractionation Mus musculus
9 Xpc  
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
Affinity Capture-MS Mus musculus
10 Zfand5  
Co-fractionation Mus musculus
11 Xpa  
Affinity Capture-Western Mus musculus
12 Rnf181  
Co-fractionation Mus musculus
13 Vcp 269523
Affinity Capture-Western Mus musculus
14 PSMD4 5710
Affinity Capture-Western Homo sapiens
15 CALM1 801
Reconstituted Complex Homo sapiens
16 Ubqln4  
Co-fractionation Mus musculus
17 PAX3  
Affinity Capture-Western Homo sapiens
18 Ngly1  
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
19 Psmd4  
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here