Gene description for U2AF1L4
Gene name U2 small nuclear RNA auxiliary factor 1-like 4
Gene symbol U2AF1L4
Other names/aliases U2AF1-RS3
U2AF1L3
U2AF1L3V1
U2AF1RS3
U2af26
Species Homo sapiens
 Database cross references - U2AF1L4
ExoCarta ExoCarta_199746
Vesiclepedia VP_199746
Entrez Gene 199746
HGNC 23020
MIM 601080
UniProt Q8WU68  
 U2AF1L4 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for U2AF1L4
Molecular Function
    pre-mRNA 3'-splice site binding GO:0030628 IBA
    metal ion binding GO:0046872 IEA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    spliceosomal complex GO:0005681 IBA
    cytoplasm GO:0005737 IEA
    nuclear speck GO:0016607 IEA
    U2AF complex GO:0089701 IBA
 Experiment description of studies that identified U2AF1L4 in sEVs
1
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for U2AF1L4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GIGYF2 26058
Affinity Capture-MS Homo sapiens
2 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
3 DYRK1A 1859
Affinity Capture-MS Homo sapiens
4 WWP1 11059
Affinity Capture-MS Homo sapiens
5 SUZ12  
Affinity Capture-MS Homo sapiens
6 PPIE 10450
Affinity Capture-MS Homo sapiens
7 U2AF1 7307
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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