Gene description for Stxbp3
Gene name syntaxin binding protein 3
Gene symbol Stxbp3
Other names/aliases Munc-18c
Stxbp3a
Sxtbp3
Unc18-3
Species Mus musculus
 Database cross references - Stxbp3
ExoCarta ExoCarta_20912
Vesiclepedia VP_20912
Entrez Gene 20912
UniProt Q60770  
 Stxbp3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Stxbp3
Molecular Function
    protein binding GO:0005515 IPI
    syntaxin-1 binding GO:0017075 IBA
    syntaxin-1 binding GO:0017075 IEA
    syntaxin-1 binding GO:0017075 ISO
    syntaxin binding GO:0019905 IDA
    syntaxin binding GO:0019905 IPI
    syntaxin binding GO:0019905 ISO
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
Biological Process
    intracellular glucose homeostasis GO:0001678 IDA
    intracellular glucose homeostasis GO:0001678 IMP
    intracellular protein transport GO:0006886 IBA
    exocytosis GO:0006887 IMP
    vesicle docking involved in exocytosis GO:0006904 IBA
    brain development GO:0007420 IMP
    protein to membrane docking GO:0022615 IMP
    insulin secretion GO:0030073 IMP
    response to insulin GO:0032868 IDA
    response to insulin GO:0032868 IMP
    neutrophil degranulation GO:0043312 IEA
    negative regulation of calcium ion-dependent exocytosis GO:0045955 IEA
    negative regulation of calcium ion-dependent exocytosis GO:0045955 ISO
    negative regulation of D-glucose import GO:0046325 IDA
    negative regulation of D-glucose import GO:0046325 IMP
    platelet aggregation GO:0070527 IEA
    platelet aggregation GO:0070527 ISO
    cellular response to type II interferon GO:0071346 IDA
    presynaptic dense core vesicle exocytosis GO:0099525 IBA
Subcellular Localization
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IPI
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    basolateral plasma membrane GO:0016323 IEA
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISO
    secretory granule GO:0030141 IBA
    platelet alpha granule GO:0031091 IEA
    platelet alpha granule GO:0031091 ISO
    specific granule GO:0042581 IEA
    specific granule GO:0042581 ISO
    phagocytic vesicle GO:0045335 IDA
    tertiary granule GO:0070820 IEA
    tertiary granule GO:0070820 ISO
 Experiment description of studies that identified Stxbp3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Stxbp3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Zbtb7b  
Affinity Capture-MS Mus musculus
2 Vamp2  
Co-fractionation Mus musculus
3 Stxbp2 20911
Co-fractionation Mus musculus
4 Snrpd2 107686
Co-fractionation Mus musculus
5 Fn1 14268
Co-fractionation Mus musculus
6 Snrpd3 67332
Co-fractionation Mus musculus
View the network image/svg+xml



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