Experiment description of studies that identified FAM171A1 in sEVs

1 Experiment ID 1203 MISEV standards ✔ Biophysical techniques ✔ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 34887515     Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name DiFi Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry RNA sequencing

2 Experiment ID 235 MISEV standards ✔ Biophysical techniques ✔ Enriched markers ✔ Negative markers ✔ Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

3 Experiment ID 237 MISEV standards ✔ Biophysical techniques ✔ Enriched markers ✔ Negative markers ✔ Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

4 Experiment ID 260 MISEV standards ✔ Biophysical techniques ✔ Enriched markers ✘ Negative markers ✔ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name 1205Lu Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 488 MISEV standards ✔ Biophysical techniques ✔ Enriched markers ✔ Negative markers ✔ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

6 Experiment ID 211 MISEV standards ✔ Biophysical techniques ✔ Enriched markers ✔ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 212 MISEV standards ✔ Biophysical techniques ✔ Enriched markers ✔ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for FAM171A1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 GJD3   Proximity Label-MS Homo sapiens 2 RAB35 11021 Proximity Label-MS Homo sapiens 3 BACE2 25825 Affinity Capture-MS Homo sapiens 4 PCDHGB1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 EBAG9 9166 Proximity Label-MS Homo sapiens 6 OCLN 100506658 Proximity Label-MS Homo sapiens 7 NRSN1   Affinity Capture-MS Homo sapiens 8 LAMTOR1 55004 Proximity Label-MS Homo sapiens 9 MARCKS 4082 Proximity Label-MS Homo sapiens 10 GJA1 2697 Proximity Label-MS Homo sapiens 11 FAM171B   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 12 PARD3 56288 Proximity Label-MS Homo sapiens 13 EPB41L4A 64097 Proximity Label-MS Homo sapiens 14 LCK 3932 Proximity Label-MS Homo sapiens 15 LGALS1 3956 Affinity Capture-MS Homo sapiens 16 CXADR 1525 Proximity Label-MS Homo sapiens 17 C11orf52 91894 Proximity Label-MS Homo sapiens 18 LYN 4067 Proximity Label-MS Homo sapiens 19 TNFRSF17   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 DNAJC5 80331 Proximity Label-MS Homo sapiens 21 MYO6 4646 Proximity Label-MS Homo sapiens 22 MLLT4 4301 Proximity Label-MS Homo sapiens 23 RASSF10   Two-hybrid Homo sapiens 24 NTRK1 4914 Affinity Capture-MS Homo sapiens 25 RAB7A 7879 Proximity Label-MS Homo sapiens 26 EPHA2 1969 Proximity Label-MS Homo sapiens 27 KRAS 3845 Proximity Label-MS Homo sapiens 28 RHOB 388 Proximity Label-MS Homo sapiens 29 DYNLL2 140735 Affinity Capture-MS Homo sapiens 30 TMEM74   Affinity Capture-MS Homo sapiens 31 CAV1 857 Proximity Label-MS Homo sapiens 32 CTDSPL 10217 Proximity Label-MS Homo sapiens 33 DYNLL1 8655 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 34 FLOT1 10211 Proximity Label-MS Homo sapiens View the network

Pathways in which FAM171A1 is involved

No pathways found