Gene description for FGF2
Gene name fibroblast growth factor 2 (basic)
Gene symbol FGF2
Other names/aliases BFGF
FGF-2
FGFB
HBGF-2
Species Homo sapiens
 Database cross references - FGF2
ExoCarta ExoCarta_2247
Vesiclepedia VP_2247
Entrez Gene 2247
HGNC 3676
MIM 134920
UniProt P09038  
 FGF2 identified in sEVs derived from the following tissue/cell type
Endothelial cells 26027894    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for FGF2
Molecular Function
    fibroblast growth factor receptor binding GO:0005104 IBA
    fibroblast growth factor receptor binding GO:0005104 IPI
    cytokine activity GO:0005125 IDA
    integrin binding GO:0005178 IDA
    protein binding GO:0005515 IPI
    growth factor activity GO:0008083 IBA
    growth factor activity GO:0008083 IDA
    heparin binding GO:0008201 IEA
    chemokine binding GO:0019956 IPI
    nuclear receptor coactivator activity GO:0030374 IDA
    chemoattractant activity GO:0042056 IDA
    identical protein binding GO:0042802 IPI
    H3K9me3 modified histone binding GO:0062072 IEA
    receptor-receptor interaction GO:0090722 IDA
Biological Process
    osteoblast differentiation GO:0001649 IEA
    branching involved in ureteric bud morphogenesis GO:0001658 IDA
    organ induction GO:0001759 IEA
    endothelial cell proliferation GO:0001935 IEA
    positive regulation of endothelial cell proliferation GO:0001938 IDA
    positive regulation of endothelial cell proliferation GO:0001938 IMP
    cell migration involved in sprouting angiogenesis GO:0002042 IDA
    cell migration involved in sprouting angiogenesis GO:0002042 IGI
    positive regulation of neuroblast proliferation GO:0002052 IEA
    transcription by RNA polymerase II GO:0006366 IEA
    chemotaxis GO:0006935 TAS
    signal transduction GO:0007165 NAS
    Ras protein signal transduction GO:0007265 TAS
    nervous system development GO:0007399 TAS
    neuroblast proliferation GO:0007405 IEA
    positive regulation of cell population proliferation GO:0008284 IBA
    positive regulation of cell population proliferation GO:0008284 IDA
    positive regulation of cell population proliferation GO:0008284 IGI
    fibroblast growth factor receptor signaling pathway GO:0008543 IBA
    fibroblast growth factor receptor signaling pathway GO:0008543 IDA
    fibroblast growth factor receptor signaling pathway GO:0008543 IGI
    fibroblast growth factor receptor signaling pathway GO:0008543 IGI
    fibroblast growth factor receptor signaling pathway GO:0008543 IPI
    embryo development ending in birth or egg hatching GO:0009792 IEA
    animal organ morphogenesis GO:0009887 TAS
    glial cell differentiation GO:0010001 IEA
    positive regulation of endothelial cell migration GO:0010595 IDA
    positive regulation of gene expression GO:0010628 IEA
    negative regulation of gene expression GO:0010629 NAS
    negative regulation of fibroblast migration GO:0010764 IDA
    growth factor dependent regulation of skeletal muscle satellite cell proliferation GO:0014843 IMP
    substantia nigra development GO:0021762 IEA
    cerebellar granule cell precursor proliferation GO:0021930 IEA
    positive regulation of cerebellar granule cell precursor proliferation GO:0021940 IEA
    neurogenesis GO:0022008 IBA
    hyaluronan catabolic process GO:0030214 IDA
    lung development GO:0030324 IEA
    regulation of cell migration GO:0030334 IBA
    paracrine signaling GO:0038001 ISS
    negative regulation of fibroblast growth factor receptor signaling pathway GO:0040037 NAS
    wound healing GO:0042060 IDA
    wound healing GO:0042060 TAS
    inner ear auditory receptor cell differentiation GO:0042491 IEA
    positive regulation of cell fate specification GO:0042660 IDA
    positive regulation of MAP kinase activity GO:0043406 IDA
    positive regulation of MAPK cascade GO:0043410 IBA
    positive regulation of MAPK cascade GO:0043410 IDA
    phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0043491 IEA
    positive regulation of blood vessel endothelial cell migration GO:0043536 IDA
    positive regulation of blood vessel endothelial cell migration GO:0043536 IGI
    negative regulation of blood vessel endothelial cell migration GO:0043537 IDA
    positive regulation of inner ear auditory receptor cell differentiation GO:0045609 IEA
    positive regulation of osteoblast differentiation GO:0045669 IEA
    regulation of angiogenesis GO:0045765 TAS
    positive regulation of angiogenesis GO:0045766 IDA
    positive regulation of angiogenesis GO:0045766 IGI
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    regulation of retinal cell programmed cell death GO:0046668 IEA
    behavioral response to ethanol GO:0048149 IEA
    embryonic morphogenesis GO:0048598 TAS
    response to axon injury GO:0048678 IEA
    stem cell development GO:0048864 IEA
    positive chemotaxis GO:0050918 IEA
    release of sequestered calcium ion into cytosol GO:0051209 IDA
    regulation of cell cycle GO:0051726 IEA
    positive regulation of cell division GO:0051781 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IDA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IGI
    positive regulation of cardiac muscle cell proliferation GO:0060045 IDA
    canonical Wnt signaling pathway GO:0060070 IEA
    corticotropin hormone secreting cell differentiation GO:0060128 IEA
    thyroid-stimulating hormone-secreting cell differentiation GO:0060129 IEA
    chondroblast differentiation GO:0060591 IDA
    mammary gland epithelial cell differentiation GO:0060644 IEA
    angiogenesis involved in coronary vascular morphogenesis GO:0060978 IEA
    negative regulation of wound healing GO:0061045 IDA
    ERK1 and ERK2 cascade GO:0070371 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IDA
    cellular response to mechanical stimulus GO:0071260 IEA
    stem cell proliferation GO:0072089 NAS
    regulation of cell migration involved in sprouting angiogenesis GO:0090049 NAS
    positive regulation of cell migration involved in sprouting angiogenesis GO:0090050 IDA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IEA
    positive regulation of lens fiber cell differentiation GO:1902748 IDA
    positive regulation of miRNA transcription GO:1902895 NAS
    positive regulation of neuroepithelial cell differentiation GO:1902913 IEA
    negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway GO:1903377 IEA
    regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis GO:1903587 NAS
    positive regulation of sprouting angiogenesis GO:1903672 IDA
    positive regulation of sprouting angiogenesis GO:1903672 IMP
    positive regulation of vascular associated smooth muscle cell proliferation GO:1904707 IGI
    lymphatic endothelial cell migration GO:1904977 IEA
    positive regulation of epithelial tube formation GO:1905278 IDA
    positive regulation of vascular endothelial cell proliferation GO:1905564 IGI
    regulation of endothelial cell chemotaxis to fibroblast growth factor GO:2000544 IDA
    regulation of endothelial cell chemotaxis to fibroblast growth factor GO:2000544 NAS
    positive regulation of endothelial cell chemotaxis to fibroblast growth factor GO:2000546 IDA
    positive regulation of DNA biosynthetic process GO:2000573 IDA
    positive regulation of DNA biosynthetic process GO:2000573 IGI
    negative regulation of stem cell proliferation GO:2000647 IEA
    positive regulation of stem cell proliferation GO:2000648 IEA
    positive regulation of stem cell differentiation GO:2000738 IEA
    positive regulation of endothelial cell chemotaxis GO:2001028 IGI
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
 Experiment description of studies that identified FGF2 in sEVs
1
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for FGF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 FGFBP1 9982
Reconstituted Complex Homo sapiens
3 SNTG1  
Reconstituted Complex Homo sapiens
4 SDC3  
Reconstituted Complex Homo sapiens
5 GPC4 2239
Reconstituted Complex Homo sapiens
6 FGFR1 2260
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Luminescence Homo sapiens
7 H3F3A 3020
Co-localization Homo sapiens
8 RPL6 6128
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
9 SDC1 6382
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
10 TUBG1 7283
Affinity Capture-MS Homo sapiens
11 VTN 7448
Reconstituted Complex Homo sapiens
12 CRYAB 1410
Protein-peptide Homo sapiens
13 TMEFF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 MEX3A  
Affinity Capture-RNA Homo sapiens
15 SDC4 6385
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
16 RPS19 6223
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
17 CSNK2A1 1457
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
18 CREBBP  
Co-localization Homo sapiens
19 SDC2 6383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
20 PRKACA 5566
Affinity Capture-Western Homo sapiens
21 FGFR2 2263
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 IL7R  
Affinity Capture-MS Homo sapiens
23 Nr4a1  
Co-localization Mus musculus
24 CSNK2A2 1459
Reconstituted Complex Homo sapiens
25 GPC3 2719
Affinity Capture-Western Homo sapiens
26 CIT 11113
Affinity Capture-MS Homo sapiens
27 THBS1 7057
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
28 CSNK2B 1460
Reconstituted Complex Homo sapiens
29 PARP1 142
Affinity Capture-MS Homo sapiens
30 CD44 960
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 COMT 1312
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which FGF2 is involved
PathwayEvidenceSource
Activated point mutants of FGFR2 TAS Reactome
Constitutive Signaling by Aberrant PI3K in Cancer TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Developmental Biology TAS Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
Disease IEA Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers IEA Reactome
Downstream signaling of activated FGFR1 TAS Reactome
Downstream signaling of activated FGFR2 TAS Reactome
Downstream signaling of activated FGFR3 TAS Reactome
Downstream signaling of activated FGFR4 TAS Reactome
Extracellular matrix organization TAS Reactome
FGFR1 ligand binding and activation TAS Reactome
FGFR1 mutant receptor activation TAS Reactome
FGFR1b ligand binding and activation TAS Reactome
FGFR1c ligand binding and activation TAS Reactome
FGFR2 ligand binding and activation TAS Reactome
FGFR2 mutant receptor activation TAS Reactome
FGFR2 mutant receptor activation IEA Reactome
FGFR2b ligand binding and activation TAS Reactome
FGFR2c ligand binding and activation TAS Reactome
FGFR3 ligand binding and activation TAS Reactome
FGFR3 mutant receptor activation TAS Reactome
FGFR3c ligand binding and activation TAS Reactome
FGFR4 ligand binding and activation TAS Reactome
FGFRL1 modulation of FGFR1 signaling TAS Reactome
Formation of intermediate mesoderm IEA Reactome
Formation of the nephric duct IEA Reactome
FRS-mediated FGFR1 signaling TAS Reactome
FRS-mediated FGFR2 signaling TAS Reactome
FRS-mediated FGFR3 signaling TAS Reactome
FRS-mediated FGFR4 signaling TAS Reactome
Gastrulation IEA Reactome
IGF1R signaling cascade TAS Reactome
Immune System TAS Reactome
Insulin receptor signalling cascade TAS Reactome
Interleukin-4 and Interleukin-13 signaling TAS Reactome
Intracellular signaling by second messengers TAS Reactome
IRS-mediated signalling TAS Reactome
IRS-related events triggered by IGF1R TAS Reactome
Kidney development IEA Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
Negative regulation of FGFR1 signaling TAS Reactome
Negative regulation of FGFR2 signaling TAS Reactome
Negative regulation of FGFR3 signaling TAS Reactome
Negative regulation of FGFR4 signaling TAS Reactome
Negative regulation of the PI3K/AKT network TAS Reactome
Non-integrin membrane-ECM interactions TAS Reactome
Phospholipase C-mediated cascade: FGFR1 TAS Reactome
Phospholipase C-mediated cascade; FGFR2 TAS Reactome
Phospholipase C-mediated cascade; FGFR3 TAS Reactome
Phospholipase C-mediated cascade; FGFR4 TAS Reactome
PI-3K cascade:FGFR1 TAS Reactome
PI-3K cascade:FGFR2 TAS Reactome
PI-3K cascade:FGFR3 TAS Reactome
PI-3K cascade:FGFR4 TAS Reactome
PI3K Cascade TAS Reactome
PI3K/AKT Signaling in Cancer TAS Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling TAS Reactome
PIP3 activates AKT signaling TAS Reactome
POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation TAS Reactome
RAF/MAP kinase cascade TAS Reactome
SHC-mediated cascade:FGFR1 TAS Reactome
SHC-mediated cascade:FGFR2 TAS Reactome
SHC-mediated cascade:FGFR3 TAS Reactome
SHC-mediated cascade:FGFR4 TAS Reactome
Signal Transduction TAS Reactome
Signaling by activated point mutants of FGFR1 TAS Reactome
Signaling by activated point mutants of FGFR3 TAS Reactome
Signaling by FGFR TAS Reactome
Signaling by FGFR in disease TAS Reactome
Signaling by FGFR in disease IEA Reactome
Signaling by FGFR1 TAS Reactome
Signaling by FGFR1 in disease TAS Reactome
Signaling by FGFR2 TAS Reactome
Signaling by FGFR2 IIIa TM IEA Reactome
Signaling by FGFR2 in disease TAS Reactome
Signaling by FGFR2 in disease IEA Reactome
Signaling by FGFR3 TAS Reactome
Signaling by FGFR3 in disease TAS Reactome
Signaling by FGFR4 TAS Reactome
Signaling by Insulin receptor TAS Reactome
Signaling by Interleukins TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by TGFB family members TAS Reactome
Signaling by TGFBR3 TAS Reactome
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R) TAS Reactome
Syndecan interactions TAS Reactome
TGFBR3 regulates FGF2 signaling TAS Reactome
Transcriptional regulation of pluripotent stem cells TAS Reactome





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